The Effect of 6 Weeks Endurance Training on the CAP1 Levels in Visceral Adipose Tissue of Male Wistar Rats with Diabetes Neuropathy

Document Type : Research Paper

Authors
Masoud Rahmati 1
Abdolreza Kazemi 2
Azam Darvishi 3
Safora Shahozadeh 4
1 Associate Professor of Exercise Physiology, Lorestan University
2 Associate Professor of Exercise Physiology, Vali E Asr University of Rafsanjan
3 Ph.D. Student of Exercise Physiology, Lorestan University
4 M.Sc. of Exercise Physiology, Islamic Azad University of Kerman
10.22089/spj.2019.6576.1826
Abstract
CAP1 is one of the structural and functional important factors in fat tissues’ abnormalities in diabetic neuropathy (DN) state. The purpose of this study was investigation of the effects of endurance training on visceral adipose tissue levels of the CAP1 in male rats with diabetic neuropathy. 30 male Wistar male rats (260±10 g) were randomly divided into three group of Control (C), Diabetic neuropathy control (DNC) and Diabetic neuropathy trained (DNT). Diabetes neuropathy induced by streptozotocin and was evaluated using behavioral pain tests. Also training group performed 6 week/ 5 sessions in week endurance training with moderate intensity. Forty-Eight hours after the last training session, subjects were anesthetized and visceral adipose tissue was removed immediately and by immunohistochemical analysis, the CAP1 protein level was measured. To assessing comparison between groups, ANOVA was used. The result of blood sugar and behavioral measurements demonstrated diabetic neuropathy model. Also, significant increase of the CAP1 levels was observed in adipose tissue of rats with diabetic neuropathy (P=0.0001) however, training was not able to change this increased level of this protein in DN state so that significant difference was not seen between DNT and DNC groups (P=0.246). The elevated levels of CAP1 may be a compensatory response to increase the glucose uptake, thermogenesis and abiogenesis. Also, the ineffectiveness of endurance training may be due to persistent hyperglycemia in the DNT group. However, the proof of these assumptions needs further studies.
Keywords
Diabetes Neuropathy
CAP1
Endurance Training
Adipose Tissue

Main Subjects

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Sport Physiology
Volume 12, Issue 46
July 2020
Pages 41-56

  • Receive Date 30 October 2018
  • Revise Date 29 January 2019
  • Accept Date 16 February 2019