the effect of 4 weeks exercise on mRNA of GDNF in rat’s hippocampus with AD induced by Aβ1-42 injection

Document Type : Research Paper

Authors
peyman ghasemi 1
reza Gharakhanlou 2
Mahdieh Molanouri Shamsi 3
davar khodadadi 4
1 Ph.D. Student of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
2 Professor of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran.
3 Associate professor of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
4 Ph.D. of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
10.22089/spj.2020.2280.1923
Abstract
Abstract: Alzheimer's disease (AD) is the most common form of dementia and amyloid
peptides playing a central role in its pathogenesis. Recently, regular exercise has been
considered as one of the most important non-pharmacological mechanisms to contrast with
AD. The aim of this study was to investigate the effect of 4 weeks exercise on mRNA of GDNF in rat’s hippocampus with AD induced by Aβ1-42 injection.
Methods: 56 male rats with mean±SD weight of 195±20 g were randomly divided
Into four groups: training group, Aβ-42 induction + training group, Aβ-42 induction group and control group. injection of Aβ1-42 into the hippocampus was performed. Seven days
after surgery, rats of each groups were either sacrificed or subjected to behavioral
testing, randomly. Real-Time PCR were used for investigation of mRNA GDNF.
Results: there is a significant difference between the groups. Gene expression level in training group was higher and in the Aβ-42 induction group was lower (p < 0.001). Moreover, spatial learning and memory were significantly better in the exercise + AD than AD group (p < 0.01).
Conclusion: It seems that 4 weeks of exercise could improve spatial memory and learning. Moreover, aerobic exercise could increase the level of neurotrophic factors gene expression specially GDNF in rat’s hippocampus.
Keywords
Alzheimer's disease
aerobic exercise
GDNF
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Sport Physiology
Volume 13, Issue 49
March 2021
Pages 169-198

  • Receive Date 13 October 2019
  • Revise Date 04 February 2020
  • Accept Date 05 February 2020