Document Type : Research Paper
Authors
- Hassan Zolfeghar Didani 1
- Momamd Reza Zolfeghar Didani 2
- Siamak Asri Rezai 3
- Firooz Ghaderi Pakdel 4
1 Ph.D. Student in Sport Physiology, Urmia University
2 Assistant Professor of Sport Physiology, Urmia University
3 Associate Professor of Biochemistry, Urmia University
4 Associate Professor of Physiology, Urmia University of Medical Sciences
Abstract
Thirty-six male wistar rats aged two months (200±8 g), were randomly divided into four groups: 1- control (12 rats), 2- Nano selenium (12 rats), 3- training (six rats) and 4- Nano selenium+ training (six rats). Then, they were supplemented with Nano selenium for 14 days (0.1 mg/kg/day) by Intraperitoneal injection. Six samples of heart tissue from groups of one and two during their resting time were collected. After two months training for groups three and four, second tissue samples were collected from all groups (in the two training groups immediately after last training session and in the other two groups during their resting time). Independent T test (P˂0.05) with SPSS21 software were used, in order to find differences in GPX, SOD, MDA and TAC between groups. First T test from first samples showed significant increase in GPX and TAC in the group two without no difference in MDA and SOD compared to the group one. Another T test between groups of one and two from second sampling indicated that, there is a significant increase in TAC in the group two compared to group one, but there were not any significant differences in other indices. The last T test from second sampling groups of three and four showed, there was a significant decrease in MDA and significant increase in GPX and TAC in group four compared to the group three, but There was not any significant difference in SOD between groups. Then short time supplementation of Nano selenium reduced the oxidative stress produced by exhausted exercise and consequently antioxidant system was amplified.
Keywords
Main Subjects
2. Fremgaard Risnes S, Hartwig A. Impact of cadmium on antioxidant enzymes in HCT116 cells and protective interaction by selenium. 2014. 10.1016/j.pisc.2014.11.016
3. Zhang J, Taylor EW, Wan X, Peng D. Impact of heat treatment on size, structure, and bioactivity of elemental Selenium nanoparticles. Int J Nanomed. 2012; 7: 815-25.
4. Turan B, Vassort G. Cardioprotective roles of Selenium in diabetes. Nutritional and therapeutic interventions for diabetes and metabolic syndrome. 2012. eBook ISBN: 9780123850843
5. Oropeza Moea M, Wisløff H, Bernhoftc A. Selenium deficiency associated porcine and human cardiomyopathies. Journal of Trace Elements in Medicine and Biology. 2015; 31: 148-56.
6. M Alissa E, M Bahijri1bdef S, A Ferns G. The controversy surrounding Selenium RA and cardiovascular disease: A review of the evidence. Med Sci Monit. 2003; 9(1): 9-18.
7. Baohua X, Zirong X, Meisheng X, Caihong H, Yue-song D, LP X. Effect of nano red elemental Selenium on GPx activity of Broiler Chick Kidney Cells in vitro. Wuhan University Journal of Nature Sgiences. 2003; 8(4): 1167-72.
8. Bansal M, Kaushal N. Managing oxidative stress/ Targeting ROS. 2014: 127-46.
9. P K, Makou R B, P D, Movahed M R S. Selenium relieves inflammation in oxidized-LDL activated platelets via p38MAPK pathway. Arak Medical University Journal (AMUJ). 2014; 17(82): 63-75. (In Persian)
10. S Mueller A, D Klomann S, M Wolf N, Schneider S, Schmidt R, Spielmann J, et al. Redox regulation of protein Tyrosine Phosphatase 1B by manipulation of dietary Selenium affects the Triglyceride concentration in rat liver. The Journal of Nutrition Biochemical, Molecular, and Genetic Mechanisms. 2008. J Nutr. 138(12):2328-36. doi: 10.3945/jn.108.089482.
11. Zendedel A, Delavari S, Ahmadvand H, Ghanadi K, Gholami M. Effects of Selenium on antioxidant activity and recovery from sciatic nerve ischemia–reperfusion in adult rats. Zahedan Journal of Research in Medical Sciences. 2015. 17( 12 ); ISSN 2383-2894.
12. Peng D, Zhang J, Liu Q, Will Taylor E. Size effect of elemental selenium nanoparticles (Nano-Se) at supranutritional levels on Selenium accumulation and glutathione S-transferase activity. Journal of Inorganic Biochemistry. 2007; 101: 1457-63.
13. Zhang J, Wang X, Xu T. Elemental selenium at nano size (nano-se) as a potential chemopreventive agent with reduced risk of Selenium toxicity: Comparison with se-methylselenocysteine in mice. Toxicological Sciences. 2008; 1: 22-31.
14. Batal A, M. Thabet N, Osman A, B. Abdel Ghaffar AR, Sh. Azab K. Amelioration of oxidative damage induced in gamma irradiated rats by nano Selenium and Lovastatin mixture. World Applied Sciences Journal. 2012; 19(7): 962-71.
15. MA Hassanin K, H Abd El-Kawi S, S Hashem K. The prospective protective effect of Selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid. International Journal of Nanomedicine. 2013; 8: 1713-20.
16. R. Ramoutar R, L. Brumaghim J. Antioxidant and anticancer properties and mechanisms of inorganic Selenium, Oxo-sulfur, and Oxo-selenium compounds. Cell Biochem Biophys. 2010; 58: 1-23.
17. Dolati V A, Mocheshi S. Muscle injury and oxidative stress following the use of Selenium supplements and exhaustive aerobic exercise in young physically-active females. J Kermanshah Univ Med Sci. 2016; 20(1); 1-5.
18. Keane Karen. Impact of HIIT (high intensity interval training) and/ or Selenium supplementation (Se) on oxidative stress and anti-oxidant status in active females. 2015;15(7):e6212 doi: 10.5867/medwave.2015.07.6212.
19. Intraperitoneal (IP) injection in rats and mice. UBC animal care guidelines. 2014.
20. Sun Lijuan, et al. Endurance exercise causes mitochondrial and oxidatives stress in rat liver. Life Sciences. 2010; 86: 34-44
21. Joseph J, Loscalzo J. Selenistasis: Epistatic effects of Selenium on cardiovascular phenotype. Nutrients. 2013; 5: 340-58.
22. L. Scheffer D, A. Silva L, B. Tromm C, L. da Rosa G, C.L. Silveira P. Impact of different resistance training protocols on muscular oxidative stress parameters. Appl Physiol Nutr Metab. 2012; 37(6):1239-1246.
23. Papp LV, Lu J, Holmgren A, Khanna KK. From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxid Redox Signal. 2007; ;9(7):775-806.
24. Khosravian Dehkordi, Kh. Effect of Selenium nano-particle on the hepatic changes in rat. World Journal of Zoology . 2014; 9 (1): 01-03.
25. Bunglavan1 S J, Garg1 A K. Dass1 and Sameer Shrivastava, R. S. Effect of supplementation of different levels of Selenium as nanoparticles/ Sodium Selenite on blood biochemical profile and humoral immunity in male Wistar rats. 2014; 1075-81.
26. Abd Allah S, S. Hashem K. Selenium nanoparticles increase the testicular antioxidant activity and spermatogenesis in male rats as compared to ordinary Selenium. International Journal of Advanced Research. 2015; 3(1): 792-802.
27. Ozkan A, Fiskin K, Gul Ayhan A. Effect of vitamin E and Selenium on antioxidant enzymes in brain, kidney and liver of cigarette smoke-exposedmice. Biologia, Bratislava. 2007; 62(3): 360-4.
28. Akil M, Gurbuz U, Bicer M, Sivrikaya A, Mogulkoc R, Kasim Baltaci A. Effect of Selenium supplementation on lipid peroxidation, antioxidant enzymes, and Lactate levels in rats immediately after acute swimming exercise. Biol Trace Elem Res. 2011; 142: 651-9.
29. Ghazanfarpoor R, Talebi E, Abedi A. Contemplation upon nano red Slenium and Sodium Selenite on antioxidant enzymes in quail under heat stress. IJSIT. 2014; 3(6): 556-565.
30. Savory L, J. Kerr C, Whiting P, Finer N, McEneny J, Ashton T. Selenium supplementation and exercise: Effect on oxidant stress in overweight adults. Obesity. 2012; 20: 794-801.
31. Omayma A.Z, EmanNoaman, A.A. Chemopreventive effect of novel nanocomposite on oxidants and antioxidant status, some liver and kidney functions and carcinoembryonic antigen against Benzo [a] pyrene induced lung carcinogenesis in mice. International Journal of Pharma Sciences. 2014; 4(4): 639-44.
32. Mohammed E.T. Safwat G.M. Assessment of the ameliorative role of selenium nanoparticles on the oxidative stress of acetaminophen in some tissues of male albino rats. 2013, 12(2) : 80-5.
33. Xun W, Shi L, Yue W, Zhang C, Ren Y, Liu Q. Effect of High-Dose Nano-selenium and Selenium–Yeast on Feed Digestibility, Rumen Fermentation, and Purine Derivatives in Sheep. December 2012, 150(3); 130–6.
)