Document Type : Research Paper
Authors
1 Associate Professor of Sport Physiology, University of Zanjan
2 M.Sc. of Applied Sport Physiology, University of Zanjan
3 Assistance Professor of Motor Behavior, University of Zanjan
Abstract
The aim of this study was to investigate the effect of 8 weeks of sprint interval training (HIIT) on antioxidant capacity, glutathione peroxidase (GPX) and lipid peroxidation, malondialdehide (MDA) of heart, liver and skeletal muscle tissues in male Wistar rats. Thirteen male Wistar rats (weight= 225.88±18.68 g, age= 8 weeks) after one-week familiarization, were randomly divided into 2 groups; Control (n=6) and sprint interval training (n=7). HIIT training protocol included 8 weeks (5 session per week) of running with 30-45 m/min on rodent treadmill (for 1 min with 10 reps and 2 min active rest) at the first week and reached to 75-80 m/min at last three weeks (for 1 min with 7 reps and 3 min active rest). GPX activity was measured by Elisa Kit and MDA level was analyzed by spectrophotometric method. Independed-samples T test results showed that the sprint interval training resulted in a significant decrease in GPX enzyme levels in heart (P=0.001), liver (P=0.002) and skeletal muscle (P=0.006) compared to the control group. Also, MDA levels of heart tissue in training group increased significantly in compare with control group (P=0.001). Based on our finding, different tissues have different oxidative responses to the similar training and HIIT imposes heart tissue on oxidative stress more than skeletal muscle and liver tissue.
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Main Subjects
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