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The Effect of Pretreatment with Interval Training and Vitamin D on IL-10، BDNF and TNFα in Brain Rat Model of Experimental Autoimmune Encephalomyelitis (EAE)

Document Type : Research Paper

Authors

1 Ph.D. in Sport Physiology, University of Mazandaran

2 Associate Professor of Sport Physiology, University of Mazandaran

3 Ph.D. Student in Sport Physiology, University of Mazandaran

Abstract

The present study aimed at investigating the pre-treatment effect of interval exercises and vitamin D supplementation on BDNF and TNFα of the brain in the rat model of experimental autoimmune encephalomyelitis (EAE). Thirty Lewis rats (six-week old) were assigned to six groups: healthy control, EAE control, EAE control D, healthy interval D, interval EAE D, and sham. Animals were subjected to 6 weeks of interval running training, 5 days in each week, which were performed 10 reps of 1-minute in each. In addition, work to rest ratio of 1: 2 and the running overall time of 30 minutes was considered. After completing the training period, the rats were immunized with guinea pig spinal cord and complete adjuvant. The disease progress and the clinical changes in rats were evaluated over 2 weeks after inducing the disease model. The results showed that there was a significant increase in IL-10 (P=0.003) and a significant decrease in TNF-α (P=0.001) in Lewis female rats with experimental autoimmune encephalomyelitis, but there was no significant change in BDNF (P=0.62). Furthermore, the clinical symptoms in trained rats were delayed. According to the results of this study, it seems that six weeks of interval training along with vitamin D supplementation can not only postpone the onset of clinical symptoms but also increase the anti-inflammatory and inflammation indices in the brain of experimental autoimmune encephalomyelitis models, and thus could help neuroprotection.

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References
  1. Smolders J, Peelen E, Thewissen M, Tervaert JWC, Menheere P, Hupperts R, et al. Safety and T cell modulating effects of high dose vitamin D 3 supplementation in multiple sclerosis. PLoS One. 2010; 5(12): 15235.
  2. Ascherio A, Munger KL, Simon KC. Vitamin D and multiple sclerosis. The Lancet Neurology. 2010; 9(6): 599-612.
  3. Simpson S, Taylor B, Blizzard L, Ponsonby AL, Pittas F, Tremlett H, et al. Higher 25‐hydroxyvitamin D is associated with lower relapse risk in multiple sclerosis. Annals of neurology. 2010 Aug 1; 68(2): 193-203.
  4. Spach KM, Hayes CE. Vitamin D3 confers protection from autoimmune encephalomyelitis only in female mice. The Journal of Immunology. 2005; 175(6): 4119-26.
  5. May E, Asadullah K, Zugel U. Immunoregulation through 1, 25-dihydroxyvitamin D 3 and its analogs. Current Drug Targets-Inflammation & Allergy. 2004; 3(4): 377-93.
  6. Pierrot-Deseilligny C, Souberbielle J-C. Contribution of vitamin D insufficiency to the pathogenesis of multiple sclerosis. Therapeutic advances in neurological disorders. 2013; 6(2): 81-116.
  7. Souza PS, Gonçalves ED, Pedroso GS, Farias HR, Junqueira SC, Marcon R, et al. Physical Exercise Attenuates Experimental Autoimmune Encephalomyelitis by Inhibiting Peripheral Immune Response and Blood-Brain Barrier Disruption. Molecular Neurobiology. 2016: 1-15.
  8. Kjølhede T, Dalgas U, Gade AB, Bjerre M, Stenager E, Petersen T, et al. Acute and chronic cytokine responses to resistance exercise and training in people with multiple sclerosis. Scandinavian journal of medicine & science in sports. 2015.
  9. Golzari Z, Shabkhiz F, Soudi S, Kordi MR, Hashemi SM. Combined exercise training reduces IFN-γ and IL-17 levels in the plasma and the supernatant of peripheral blood mononuclear cells in women with multiple sclerosis. International immunopharmacology. 2010; 10(11): 1415-9.
  10. Rossi S, Furlan R, De Chiara V, Musella A, Giudice TL, Mataluni G, et al. Exercise attenuates the clinical, synaptic and dendritic abnormalities of experimental autoimmune encephalomyelitis. Neurobiology of disease. 2009; 36(1): 51-9.
  11. Huang Y, Dreyfus CF. The role of growth factors as a therapeutic approach to demyelinating disease. Experimental neurology. 2016; 283: 531-40.
  12. Stadelmann C, Kerschensteiner M, Misgeld T, Brück W, Hohlfeld R, Lassmann H. BDNF and gp145trkB in multiple sclerosis brain lesions: neuroprotective interactions between immune and neuronal cells? Brain. 2002; 125(1): 75-85.
  13. Zhu W, Frost EE, Begum F, Vora P, Au K, Gong Y, et al. The role of dorsal root ganglia activation and brain‐derived neurotrophic factor in multiple sclerosis. Journal of cellular and molecular medicine. 2012; 16(8): 1856-65.
  14. Sarchielli P, Greco L, Stipa A, Floridi A, Gallai V. Brain-derived neurotrophic factor in patients with multiple sclerosis. Journal of neuroimmunology. 2002; 132(1): 180-8.
  15. Azoulay D, Urshansky N, Karni A. Low and dysregulated BDNF secretion from immune cells of MS patients is related to reduced neuroprotection. Journal of neuroimmunology. 2008; 195(1): 186-93.
  16. Azoulay D, Vachapova V, Shihman B, Miler A, Karni A. Lower brain-derived neurotrophic factor in serum of relapsing remitting MS: reversal by glatiramer acetate. Journal of neuroimmunology. 2005; 167(1): 215-8.
  17. Gielen A, Khademi M, Muhallab S, Olsson T, Piehl F. Increased Brain‐Derived Neurotrophic Factor Expression in White Blood Cells of Relapsing–Remitting Multiple Sclerosis Patients. Scandinavian journal of immunology. 2003; 57(5): 493-7.
  18. Petereit H, Lindemann H, Schoppe S. Effect of immunomodulatory drugs on in vitro production of brain-derived neurotrophic factor. Multiple Sclerosis Journal. 2003; 9(1): 16-20.
  19. Marquez CMS, Vanaudenaerde B, Troosters T, Wenderoth N. High-intensity interval training evokes larger serum BDNF levels compared with intense continuous exercise. Journal of Applied Physiology. 2015; 119(12): 1363-73.
  20. Ogura Y, Naito H, Kurosaka M, Sugiura T, Junichiro A, Katamoto S. Sprint-interval training induces heat shock protein 72 in rat skeletal muscles. Journal of sports science & medicine. 2006; 5(2): 19. 4.
  21. Yaghmaei P NM, Mahdavi M,Mehrvarz T, Zahra Nazari. The effect of different doses of bee venom serum levels of interleukin-6 in Lewis rats EAE (a model for studying multiple sclerosis). Pajoohandeh Journal. 2013: 18 (2): 69-75. (In Persian).
  22. Mosayebi G GA, payani M. A. The Effect of Vitamin D3 on the Inhibition of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice. Razi Journal of Medical Sciences. 2007: 13(52),189-96. (In Persian).
  23. Patel DI, White LJ. Effect of 10-day forced treadmill training on neurotrophic factors in experimental autoimmune encephalomyelitis. Applied Physiology, Nutrition, and Metabolism. 2013; 38(2): 194-9.
  24. Castellano V, White LJ. Serum brain-derived neurotrophic factor response to aerobic exercise in multiple sclerosis. Journal of the neurological sciences. 2008; 269(1): 85-91.
  25. Yarrow JF, White LJ, McCoy SC, Borst SE. Training augments resistance exercise induced elevation of circulating brain derived neurotrophic factor (BDNF). Neuroscience letters. 2010; 479(2): 161-5.
  26. Ploughman M, Granter-Button S, Chernenko G, Tucker B, Mearow K, Corbett D. Endurance exercise regimens induce differential effects on brain-derived neurotrophic factor, synapsin-I and insulin-like growth factor I after focal ischemia. Neuroscience. 2005; 136(4): 991-1001.
  27. Klaren RE, Stasula U, Steelman AJ, Hernandez J, Pence BD, Woods JA, et al. Effects of exercise in a relapsing‐remitting model of experimental autoimmune encephalomyelitis. Journal of Neuroscience Research. 2016; 94(10): 907-14.
  28. Huang A, Jen C, Chen H, Yu L, Kuo Y, Chen H-I. Compulsive exercise acutely upregulates rat hippocampal brain-derived neurotrophic factor. Journal of neural transmission. 2006; 113(7): 803-11.
  29. Wu A, Ying Z, Gomez-Pinilla F. Docosahexaenoic acid dietary supplementation enhances the effects of exercise on synaptic plasticity and cognition. Neuroscience. 2008; 155(3): 751-9.
  30. Maimone D, Gregory S, Arnason BG, Reder AT. Cytokine levels in the cerebrospinal fluid and serum of patients with multiple sclerosis. Journal of neuroimmunology. 1991; 32(1): 67-74.
  31. Bernardes D, Oliveira-Lima OC, da Silva TV, Faraco CCF, Leite HR, Juliano MA, et al. Differential brain and spinal cord cytokine and BDNF levels in experimental autoimmune encephalomyelitis are modulated by prior and regular exercise. Journal of neuroimmunology. 2013; 264(1): 24-34.
  32. Hauser SL, Oksenberg JR. The neurobiology of multiple sclerosis: genes, inflammation, and neurodegeneration. Neuron. 2006; 52(1): 61-76.
  33. Das UN. Anti-inflammatory nature of exercise. Nutrition. 2004; 20(3): 323.
  34. Bernardes D, Brambilla R, Bracchi‐Ricard V, Karmally S, Dellarole A, Carvalho‐Tavares J, et al. Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis. Journal of neurochemistry. 2016; 136(S1): 63-73.
  35. Patanella AK, Zinno M, Quaranta D, Nociti V, Frisullo G, Gainotti G, et al. Correlations between peripheral blood mononuclear cell production of BDNF, TNF‐alpha, IL‐6, IL‐10 and cognitive performances in multiple sclerosis patients. Journal of neuroscience research. 2010; 88(5): 1106-12.
  36. Lemire J. 1, 25-Dihydroxyvitamin D 3-a hormone with immunomodulatory properties. Zeitschrift für Rheumatologie. 2000; 59(7): 117-24. 
Sport Physiology
Volume 9, Issue 36 - Serial Number 36
Winter 2018
January 2018
Pages 129-142
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History
  • Receive Date: 17 June 2017
  • Revise Date: 10 October 2017
  • Accept Date: 23 October 2017
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