Document Type : Research Paper
Authors
1 Ph.D Student of Sport Physiology, Lorestan University
2 Assistant Professor of Sport Physiology, Lorestan University
3 Associate Professor of Sport Physiology, Lorestan University
Abstract
The aim of this study was to investigate the effect of endurance training on XBP-1 and ATF6 proteins content in sciatica tissue of diabetic male wistar rats. The experimental design was a post-test design with control group and conducted in a laboratory. In this study, 32 rats 10 weeks old with an average weight of 253.16 ± 10.16 gr were randomly divided into four groups: healthy control, healthy training, diabetic control and diabetic training group. An intraperitoneal injection of STZ (45 mg/kg) solution was used to induce diabetes after 12 hours of fasting. 48 hours after STZ injection, the moderate-intensity endurance protocol was performed for six weeks. Twenty-four hours after the last training session, the rats were exposed and their sciatic nerve was extracted. The amount of XBP-1 and ATF6 proteins were measured by ELISA method. For statistical analysis, SPSS software and two-way ANOVA with repeated measure and independent t test were used for post hoc test. Data analysis showed that endurance training significantly reduced the level of XBP-1 (P = 0.007) and ATF6 (P = 0.001) proteins in the diabetic training group compared with the control diabetes group .The levels of XBP-1 and ATF6 proteins, which are likely to contribute to the progression of diabetes and nerve damage, Endurance training has been reduced in healthy rats and diabetics, which can help prevent the nerve damage.
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Main Subjects
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