Document Type : Research Paper
Authors
1 Assistant Professor of Exercise Physiology, Urmia University
2 Ph.D. Student of Exercise Physiology, Urmia University
3 Assistant Professor of Clinical Biochemistry, Tabriz University
Abstract
Weakness, intolerance of exercise and eventually muscle atrophy are important features of patients with heart failure. Therefore, the purpose of this study was to investigate the effect of 8 weeks of high intensity interval training on ubiquitin-proteasome system in fast twitch muscle of post myocardial infarction (MI) rats. Methods: Myocardial infarction was induced by left artery descending ligation for 30 min. 32 male Wistar rats were randomly divided into 4 groups (n = 8): MI control (MI-CTL), high-intensity interval MI (MI-HIIT), high-intensity interval (HIIT) and Sham- operated. Exercise training was undergoing 40 min, 3 sessions per week for 8-weeks (85-90% VO2max). 24 hours after the last training session, the rats were anesthetized and their extensor digitorum longus (EDL) tissue removed. RT-PCR method was used to measuregene expression (NOX2, TNF-a, NF-KB, P38 MAPK, Murf-1) was used. Data analysis were performed using a one-way ANOVA and Tukey follow up tests, considering as significance level (P < 0.05). Results: 8-week HIIT significantly decreased NOX2, TNF-a in MI-HIIT compared with MI-CTL and Sham (P=0.03). These reductions wereparalleled a significant decrease in activity NF-KB, P38, Murf-1 in MI-HIIT compared with MI-CTL and Sham (P<0.05). Also, NOX2 change in HIIT group was significantly higher than MI-CTL and Sham (P<0.05). According to the results of HIIT probably as an effective treatment, it reduces muscle atrophy in fast muscle contraction of heart patients.
Keywords
- High- Intensity Interval Training
- Ubiquitin-Proteasome System
- Myocardial Infarction
- Fast Twitch Muscle
Main Subjects
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