Document Type : Research Paper
Authors
1 Associate Professor of Exercise Physiology, Razi University
2 Ph.D. Student of Exercise Physiology, Razi University
Abstract
The purpose of this study was effect of eight weeks of resistance training with citrulline-malate supplementation on blood pressure, serum nitric oxide and vascular endothelial growth factor in in rest rate and response to physical activity in postmenopausal women with prehypertension. 42 postmenopausal women (Age: 53.17 ± 1.82-year, height: 158.91 ± 1.86 centimeter, BMI: 28.79 ± 1.71 kg/m2) with prehypertension (with an average systolic blood pressure of 120 to 139 mmHg) were randomly assigned into six groups: exercise + supplements, exercise + placebo, exercise, untrained, supplemented, supplemented, and placebo. Resistance training includes 7 moves and was performed three times, every move three sets of 12-10 reps for week with intensity %45-%55 of 1RM for 8 weeks. Supplementation included 8 grams of Citrulline-Malate one hour before exercise. Measurements in 4 times before and after exercise, blood pressure, NO and VEGF were measured using a barometer and kit. ANOVA test, Tukey post-hoc test and dependent t-test were used for analyzing the data at a significantly level 0.05. Two-way ANOVA and Tukey post hoc tests were used for the significant level (P < 0.05). The results showed that consumption of citrulline-malate was effective in decreasing systolic, diastolic blood pressure and NO in response to endurance activity. However, eight weeks of training had no significantly effect on the reduction of systolic, diastolic and increased NO and VEGF in response to endurance activity. Also, resistance training with citrulline-malate as effective in decreasing systolic, diastolic blood pressure, and increased NO and VEGF in response to endurance activity. It seems that the effect of exercise+ supplements on the reduction of systolic and diastolic blood pressure in response to submaximal endurance activity, these favorable effects were associated with an increase in NO and VEGF levels.
Keywords
- Citrulline-Malate
- Nitric Oxide (NO)
- Physical Activity
- Vascular Endothelial Growth Factor (VEGF)
- Prehypertensive Menopausal Women
Main Subjects
- Haring B, Liu J, Salmoirago-Blotcher E, Hayden KM, Sarto G, Roussouw J, et al. Blood pressure variability and brain morphology in elderly women without cardiovascular disease. Neurology. 2019;92(12):1284-97.
- Tikhonoff V, Casiglia E, Gasparotti F, Spinella P. The uncertain effect of menopause on blood pressure. human hypertension. 2019;33(6):421-8.
- Shimojo GL, Silva Dias DD, Malfitano C, Sanches IC, Llesuy S, Ulloa L, Irigoyen MC, De Angelis K. Combined aerobic and resistance exercise training improve hypertension associated with menopause. Frontiers in physiology. 2018 Oct 29; 9:1471.
- Zaheri S, Tadibi V, Monazami A, Naleini F. Acute effects of an interval aerobic session on flow-mediated dilation and blood pressure in women with prehypertension. Sport Physiology, 2017;9(34):35-48. (In Persian).
- Soori R, Khosravi N, Mirshafiey S, Gholijani F, Rezaeian N. Effects of resistance training on angiopoietin-like protein 4 and lipids profile levels in postmenopausal obese women. Sport Physiology. 2018; 9(36):39-58. (In Persian).
- Chiba T, Sakuma K, Komatsu T, Cao X, Aimoto M, Nagasawa Y, et al. Physiological role of nitric oxide for regulation of arterial stiffness in anesthetized rabbits. pharmacological sciences. 2019;139(1):42-5.
- Botchlett R, Lawler JM, Wu G. L-Arginine and l-citrulline in sports nutrition and health. In Nutrition and enhanced sports performance; 2019; 1 (pp. 645-52).
- Houston M, Hays L. Acute effects of an oral nitric oxide supplement on blood pressure, endothelial function, and vascular compliance in hypertensive patients. Clinical Hypertension, 2014; 16(7):524-9.
- Mas-Capdevila A, Iglesias-Carres L, Arola-Arnal A, Aragonès G, Aleixandre A, Bravo FI, Muguerza B. Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats. Nutrients. 2019; 11(2): 225-32.
- Barkhidarian B, Khorshidi M, Shab-Bidar S, Hashemi B. Effects of L-citrulline supplementation on blood pressure: A systematic review and meta-analysis. Avicenna journal of phytomedicine. 2019;9(1):10-25.
- Casonatto J, Cavalari JV, Goessler KF, Christofaro DG, Polito MD, Enokida DM, Grandolfi K. Citrulline malate supplementation might potentiate post-exercise hypotension in hypertensives: A 24-hour analysis. Science & Sports. 2019 1;34(4):261-e1.
- Mahboobi S, Tsang C, Rezaei S, Jafarnejad S. Effect of L-citrulline supplementation on blood pressure: A systematic review and meta-analysis of randomized controlled trials. human hypertension, 2019;33(1):10-21.
- Papadia C, Osowska S, Cynober L, Forbes A. Citrulline in health and disease. Review on human studies. Clinical Nutrition. 2018;37(6 Pt A):1823-8.
- Powers R, Weissgerber TL, McGonigal S, Myerski A, Gallaher M, Speer PD, et al. L-Citrulline administration increases the arginine/ADMA ratio, decreases blood pressure and improves vascular function in obese pregnant women. Pregnancy Hypertension: Women's Cardiovascular Health. 2015;5(1):4-11.
- Dong JY1, Qin LQ, Zhang Z, Zhao Y, Wang J, Arigoni F, et al. Effect of oral L-arginine supplementation on blood pressure: A meta-analysis of randomized, double-blind, placebo-controlled trials article in American heart journal. 2011;162(6); 959-65.
- Figueroa A, Wong A, Jaime SJ, Gonzales JU. Influence of L-citrulline and watermelon supplementation on vascular function and exercise performance. Current opinion in clinical nutrition and metabolic care. 2017;20(1):92-8.
- Bjarnegård N, Hedman K, Länne T. Vascular adaptation to indoor cycling exercise in premenopausal women. sports medicine. 2019;40(04):245-52.
- Figueroa A, Okamoto T, Jaime SJ, Fahs CA. Impact of high-and low-intensity resistance training on arterial stiffness and blood pressure in adults across the lifespan: A review. Pflügers Archiv-European Physiology. 2019;471(3):467-78.
- Takeda K, Takemasa T. An Overview of Ornithine, Arginine, and Citrulline in Exercise and Sports Nutrition. In Nutrition and Enhanced Sports Performance 2019 1 (pp. 627-36).
- Sureda A, Pons A. Arginine and citrulline supplementation in sports and exercise: ergogenic nutrients? Med Sport Sci. 2012; 59:18-28.
- Ranjbar K, Nurshahi M, Hedayati M, Taheri Chadoranshin H. Evaluation of serum levels of angiogenic factors in response to a long-term suboptimal activity in inactive men. Pharmacology and Pharmacology; 2012; 15:124-32.
- Wax B, Kavazis AN, Luckett W. Effects of supplemental citrulline-malate ingestion on blood lactate, cardiovascular dynamics, and resistance exercise performance in trained males. Journal of dietary supplements. 2016;13(3):269-82.
- Mirenayat MS, Moradi S, Mohammadi H, Rouhani MH. Effect of L-citrulline supplementation on blood pressure: A systematic review and meta-analysis of clinical trials. Current hypertension reports. 2018;20(11):98-112.
- Troncoso, LJ. The effects of supplemental citrulline malate on arterial elasticity, aerobic performance, blood pressure, and heart rate. In International Journal of Exercise Science: Conference Proceedings. 2017;2(9),78-88.
- Wax B, Kavazis AN, Weldon K, Sperlak J. Effects of supplemental citrulline malate ingestion during repeated bouts of lower-body exercise in advanced weightlifters. J Strength Cond Res. 2015;29(3):786-92.
- Douglas KS, Jeferson LJ, Walquiria BA, Mirela C, Roveratti MC, Estoche JM, et al. Citrulline malate does not improve muscle recovery after resistance exercise in untrained young adult men. Nutrients. 2017; 9(10):1132-48.
- Shah AR, Van Horn AN, Verchinina L, Wichorek M, Su L, Markel D, Gardner TW. Blood pressure is associated with receiving intravitreal anti–vascular endothelial growth factor treatment in patients with diabetes. Ophthalmology Retina. 2019;3(5):410-6.
- Mehri Alvar, Y., Sayeevand, Z., Erfani Adab, F., Heidari Moghadam, R., Samavat Sharif, M., Karami, S. The effects of five weeks resistance training on some vascular growth factors in sedentary men. Sport Physiology. 2016;8(29):15-30.
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