تأثیر ترکیب تمرین تناوبی با شدت بالا و روزه‌داری متناوب بر مقادیر پروتئینی PGC-1α و UCP-1 بافت چربی احشایی رت های تحت تغذیه با رژیم غذایی پرچرب و پرکربوهیدرات

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان ایران

2 مرکز تحقیقات فیزیولوژی، دانشگاه علوم پزشکی ایران، تهران، ایران.

10.22089/spj.2024.17238.2334

چکیده

اهداف: مداخلات تغذیه‌ای و فعالیت ورزشی از راهبردهای اصلی مقابله‌ای و درمان چاقی است. پژوهش حاضر با هدف یافتن جنبه جدیدی از تأثیر تمرین تناوبی با شدت بالا (HIIT) و روزه‌داری متناوب (ADF) بر مقادیر پروتئین‌های مرتبط با قهوه‌ای شدن بافت چربی احشایی رت‌های تحت تغذیه با رژیم غذایی پرچرب و پرکربوهیدرات (HFD+HC) انجام شد. مواد و روش‌ها: روش پژوهش حاضر از نوع تجربی، با طرح پس‌آزمون با کنترل بود. بدین منظور 40 سر رت نر اسپراگ‌داولی به‌طور تصادفی به پنج گروه هشت‌تایی تقسیم شدند: 1) رژیم غذایی استاندارد (ND)، 2) HFD+HC، 3) HFD+HC+HIIT، 4) HFD+HC+ADF و 5) HFD+HC+HIIT+ADF. رت‌های HIIT پنج روز در هفته و در هفت تناوب چهار دقیقه‌ای با90ـ85 درصد حداکثر سرعت (Smax) و تناوب‌های استراحتی دو دقیقه‌ای با 50 درصد Smax دویدند. ADF به‌صورت یک روز در میان انجام می‌شد. مقادیر پروتئین PGC-1α و UCP-1 بافت چربی با تکنیک وسترن بلات ارزیابی شد. از t مستقل، ANOVA و آزمون توکی برای تجزیه و تحلیل داده‌ها استفاده شد. یافته‌ها: HFD+HC منجر به افزایش معنی‌دار وزن شد. مقادیر پروتئین‌های PGC-1α و UCP-1 در گروه HFD+HC کاهش معنی‌داری یافت (05/0p<). در گروه‌های HIIT، ADF و HIIT+ADF وزن کاهش معنی‌داری یافت. در گروه-های HIIT و HIIT+ADF مقادیر پروتئینی PGC-1α افزایش معنی‌داری یافت (05/0p<). با این حال، مقادیر پروتئینی UCP-1 تفاوت معنی‌داری بین گروه‌های مداخله نداشت (05/0p>). نتیجه‌گیری: HFD+HC منجر به افزایش وزن می‌شود. در مقابل، HIIT، ADF و به‌ویژه HIIT+ADF علاوه‌بر کاهش وزن منجر به افزایش مقادیر پروتئین PGC-1α می‌شود که احتمالاً با بیوژنز میتوکندریایی و افزایش لیپولیز همراه است.

کلیدواژه‌ها

موضوعات

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فیزیولوژی ورزشی
دوره 16، شماره 61
فروردین 1403
صفحه 135-152

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  • تاریخ دریافت: 25 شهریور 1403
  • تاریخ بازنگری: 11 آذر 1403
  • تاریخ پذیرش: 20 آذر 1403

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