تجزیه‌ و تحلیل سیستمی سازوکارهای مولکولی فعالیت ورزشی در تنظیم بیوسنتز کلسترول: مدل‌سازی شبکه‌های مولکولی برای شناسایی اهداف درمانی

عبداللهی, میلاد; مرندی, سید محمد; صفائی نژاد, زهرا; نصر اصفهانی, محمدحسین

doi:10.22089/spj.2025.17867.2363

تجزیه‌ و تحلیل سیستمی سازوکارهای مولکولی فعالیت ورزشی در تنظیم بیوسنتز کلسترول: مدل‌سازی شبکه‌های مولکولی برای شناسایی اهداف درمانی

نوع مقاله : مقاله پژوهشی

نویسندگان

میلاد عبداللهی ¹

سید محمد مرندی ¹

زهرا صفائی نژاد ²

محمدحسین نصر اصفهانی ²

¹ گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

² گروه ژنتیک، مؤسسه رویان اصفهان، اصفهان، ایران

10.22089/spj.2025.17867.2363

چکیده

تنظیم بیوسنتز کلسترول فرایندی پیچیده و به‌شدت کنترل‌شده است که شامل شبکه‌ای از ژن‌ها می‌شود که سنتز، تنظیم و هومئوستاز سطح کلسترول را هماهنگ و تنظیم می‌کنند. در این مطالعه به تحلیل ژن‌های کلیدی مانند Idi1، Fdps، Sqle، Hmgcs1 پرداخته شده است که در بیوسنتز کلسترول، متابولیسم لیپیدها و سیگنالینگ سلولی نقش‌های اساسی دارند. مدل‌سازی محاسباتی این شبکه‌های تنظیمی می‌تواند تعاملات پیچیده بین این ژن‌ها و مکانیزم‌های تنظیمی آن‌ها را در شرایط مختلف ازجمله رژیم‌های پرچرب، نمایان کند. تحلیل بیوانفورماتیکی ما تأثیر رژیم غذایی پرچرب و فعالیت ورزشی بر این ژن‌ها را بررسی کرد و به بررسی تنظیمات دینامیک مسیرهای بیوسنتز کلسترول و قابلیت تنظیم آن‌ها پرداخت. علاوه بر این، شناسایی اهداف دارویی جدید مانند MicroRNAها می‌تواند به استراتژی‌های درمانی نوآورانه برای مدیریت دیس لیپیدمی منجر شود. با ترکیب رویکردهای زیست‌شناسی سیستمی و ابزارهای محاسباتی، هدف مطالعه حاضر این بود تا درک بهتری از متابولیسم کلسترول به دست آورد و تأثیر فعالیت ورزشی و مداخلات دارویی جدیدی را برای اختلالات لیپیدی شناسایی کند. یافته‌ها نشان داد، مسیرهای بیوسنتز کلسترول با استفاده از داده‌های رونویسی تحلیل شده و ژن‌های هاب درگیر در این مسیرها شناسایی شدند. همچنین تجزیه ‌و تحلیل خوشه‌ای شبکه نتیجه تحلیل‌های GO و KEGG را تأیید کرد. نتیجه‌گیری می‌شود، این مطالعه فهرستی از ژن‌های هاب، مسیرهای مهم و بینش‌های جدید برای توسعه استراتژی‌های مدیریت بیوسنتز کلسترول را ارائه کرده و مکانیسم‌های اساسی پاسخ کبد را روشن می‌کند.

کلیدواژه‌ها

بیوسنتز کلسترول

شبکه‌های تنظیمی ژنی

دیس لیپیدمی

فعالیت ورزشی

بیوانفورماتیک

موضوعات

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