تأثیر ترکیب تمرین هوازی و مکمل اسپیرولینا بر میزان بیان ژن‌های مسیر سیگنالینگ BDNF/p75NTR در رت‌های نر دیابتی نژاد ویستار

جعفریان, فاطمه; رضایی, زینب; مرندی, سید محمد; راداحمدی, مریم

doi:10.22089/spj.2025.18014.2372

تأثیر ترکیب تمرین هوازی و مکمل اسپیرولینا بر میزان بیان ژن‌های مسیر سیگنالینگ BDNF/p75NTR در رت‌های نر دیابتی نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

فاطمه جعفریان ¹

زینب رضایی ¹

سید محمد مرندی ¹

مریم راداحمدی ²

¹ گروه فیزیولوژی ورزش، دانشکده علوم ورزشی دانشگاه اصفهان، اصفهان. ایران

² گروه فیزیولوژی، دانشکده پزشکی دانشگاه علوم پزشکی اصفهان، اصفهان. ایران

10.22089/spj.2025.18014.2372

چکیده

هدف: دیابت به‌عنوان اختلال متابولیک خاص مغز شناخته شده است که نقش حیاتی در تخریب نورونی دیابتی دارد. اصلاح سبک زندگی سبب بهبود و کنترل اختلالات این بیماری پیشرونده و شایع می‌شود. در این مطالعه اثر چهار هفته ورزش هوازی و مکمل اسپیرولینا بر مسیر سیگنالینگ BDNF/p75 در رتهای دیابتی مدل استرپتوزوسین بررسی شد.
مواد و روش ها: تعداد 28 سر رت نر نژاد ویستار پس از تزریقmg/kg 50 استرپتوزوسین به صورت داخل صفاقی و ارزیابی گلوکز خون ناشتا برای تأیید ایجاد مدل دیابت، به چهار گروه تقسیم شدند: گروه کنترل دیابت؛ گروه دیابت+اسپیرولینا؛ گروه دیابت+ورزش؛ و گروه دیابت+ورزش+اسپیرولینا. گلوکز خون ناشتا در بازه‌های زمانی دو هفته و چهار هفته پس از القای دیابت بررسی شد. همچنین 72 ساعت پس از آخرین جلسه تمرینی بیان ژن‌های BDNF و p75 در هیپوکمپ رت‌ها از طریق روش RT-PCR بررسی شد. تحلیل‌های آماری با روش آنوای یک‌طرفه در نرم‌افزار SPSS انجام شد.
یافته ها: نتایج نشان داد، روش‌های مداخلهای پس از شش هفته سبب کاهش معنادار گلوکز خون و بیان P75 در مقایسه با گروه کنترل دیابت شد و بیان BDNF به طور معناداری افزایش یافت (0.01P≤). اختلاف بین گروههای تک‌مداخلهای معنادار نشد (0.05P>)، اما در گروه مداخله ترکیبی اختلاف در فاکتورهای بررسی‌شده در مقایسه با سایر گروهها معنادار شد (0.01P≤).
نتیجه گیری: نتیجه گرفته می‌شود که کنترل متابولیک و بهبود حفاظت نورونی به دنبال فعالیت ورزشی و دریافت مکمل اسپیرولینا در رت‌های دیابتی می‌تواند سبب بهبود اختلالات شود.

کلیدواژه‌ها

اسپیرولینا

تمرین هوازی

گلوکز خون

فاکتور تغذیه‌ای مشتق از مغز

موضوعات

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