Document Type : Research Paper

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Abstract

In this study, we investigate the effect of treadmill running with consumption of 1.25 dihydroxyvitamin D3 on NGF levels in Parkinsonian rats’ striatum induced by 6-hydroxy-dopamine. 48 rats (weight 276± 10g and age 12 week) were divided into 6 groups: control, sham، control Parkinson, exercise + Parkinson, vitamin D3 + Parkinson, vitamin D3 + exercise + Parkinson. Exercise group: five days per week for 4 weeks during the light cycle. Rats were run twice per day at a speed of 15 m/min separated by at least 1 hr; each running bout lasted 15 min for a total of 30 min per day. Parkinson supplement group in during study period received 1 µg/ml/kg VD3 intraperitoneally Two times per week. To induce Parkinson, 250 mg/kg in volume 5m/l of 6-hydroxydopamine (6-OHDA) was administered into striatum by a stereotaxic apparatus. NGF level in the striatum were measured by ELISA. Alone-intensity exercise the protocol presented in this study, nerve growth factor levels Increased but not significant(P=0.372). On the other, the supplementation VD3 alone levels of nerve growth factor (P=0.002) to increase significantly، also combining it with exercise could NGF levels (P=0.0001) shows a significant increase. Our findings show that pre-treatment with treadmill running and consumption supplement of 1.25 dihydroxyvitamin D3 have a protective role against 6-OHDA induced toxicity.

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