Effect of L-Arginine supplementation on nAchR response in skeletal muscles to exhaustive acute exercise in old rats

Document Type : Research Paper

Authors

1 Associate Professor of Sport Physiology, Shahid Beheshti University

2 Ph. D. Student of Sport Physiology, Shahid Beheshti University

3 M. Sc. of Sport Physiology, Shahid Beheshti University

4 Assistant Professor of Sport Physiology, Shahid Rajaee Teacher Training University

5 Associate Professor of Neuroscience Research Center, Shahid Beheshti University of Medical Sciences

Abstract

The purpose of this study was to investigate the effect of L-arginine supplementation on the nAchR response in hind limb of old male rats after exhaustive acute exercise. For this purpose, 32 Wistar rats (age: 22 months, weight: 380±20gr) were selected. After one week of familiarization with laboratory environment, they were randomly divided into four groups consisted of:1) L-arginine supplement with one session of exhaustive endurance exercise, 2) L-arginine supplement 3) exercise and 4) control group. For the supplementation groups, they consumed water containing 2.5% L-arginine for two weeks. In the exercise day, the training groups performed an exhaustive running with 30 meter per minute speed and 5-degree incline, equivalent to approximately 75-80% of their VO2max. Immediately and four hours after exercise they anesthesia and sampling was performed from EDL and Soleus muscles. Then, for statistical analysis of the data's, the one-way ANOVA was used. data of this study illustrate that nAchR level in L-arginine supplement, significantly was higher than control group in EDL and Soleus muscles (p< 0/001). And also nAchR level in L-arginine supplement with exercise and exercise group was increased significantly four hours after exhaustive exercise than control group in EDL and Soleus muscles (p< 0/001). The result showed that L-arginine supplementation with exercise increased of nAchR levels in old rat. It seems L-arginine supplementation with exercise can be an appropriate approach to decrease Sarcopenia with effect on nAchR level in old aged people.

Keywords

Main Subjects

References

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Sport Physiology
Volume 9, Issue 33
May 2017
Pages 169-184

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History

  • Receive Date: 14 February 2016
  • Revise Date: 15 May 2016
  • Accept Date: 21 May 2016

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