Effect of Aerobic Training and Eugenol on the Expression of SERCA2a and NKX2-5 Genes in Heart Tissue of Rats Poisoned with Chlorpyrifos

Document Type : Research Paper

Authors
Alibemani Ranjbar 1
Hasan Matinhomaee 2
Saleh Rahmati-Ahmadabad 3
1 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

3 Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran.

10.22089/spj.2021.10142.2119
Abstract
Chlorpyrifos is an organophosphate toxin used to control agricultural pests. These toxins cause oxidative stress and apoptosis in various tissues of the body, including the heart. SERCA2a and NKX2-5 are genes that regulate contractile function and heart growth. Previous studies have shown a positive effect of physical activity on the expression of these genes in heart failure, but so far, changes in these genes following the use of physical activity in chlorpyrifos poisoning have not been studied. The aim of the present study was to investigate the independent and interactive effect of aerobic exercise and eugenol supplementation on the expression of SERCA2a and NKX2-5 genes in the heart tissue of rats poisoned with chlorpyrifos. Thirty-six Wistar male rats were divided into six groups (six in each group) with the names healthy control, sham, poison control, aerobic exercise poison, eugenol poison, and toxic + aerobic exercise + eugenol. Aerobic training was performed on a rodent treadmill (five sessions per week, each session lasting 20 minutes at a speed of 26 meters per minute) for four weeks. Eugenol at a dose of 250 mg/kg body weight was fed to the rats of the supplement groups (five days a week for four weeks using gavage). Data were analyzed using one-way ANOVA with Tukey post-hoc test and P <0.05 was considered as a significant change. The results of the current study showed that poisoning significantly reduced the expression of SERCA2a and NKX2-5 genes in cardiac tissue. Exercise (no supplementation) increased the SERCA2a and NKX2-5 genes in the hearts of poisoned rats. Exercise-eugenol interaction did not cause a synergistic effect. Overall, in the present study, exercise seems to be a more important factor than eugenol in reducing the effect of chronic intoxication on the heart. Further studies with higher doses of eugenol supplementation are needed.
Keywords
Oxidative stress
apoptosis
organophosphate toxins
physical activity
poisoning
eugenol supplement
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Sport Physiology
Volume 13, Issue 51 - Serial Number 51
November 2021
Pages 139-158

  • Receive Date 28 February 2021
  • Revise Date 28 August 2021
  • Accept Date 30 August 2021