Ceramides are a family of fat molecules that play an important role in glucose homeostasis and insulin signaling. The aim of this study was to compare the effect of two methods of resistance training on ceramide metabolism and insulin sensitivity in overweight and obese young men. 33 overweight and obese men (age: 17.7 ± 0.13 years, weight: 92 ± 2.45 kg, BMI: 30.70 ± 1.32 kg/m2) were selected and randomly divided into three groups. The training program consisted of 12 weeks of traditional and circular resistance training that was exactly the same size and pattern as the wave, which was performed three sessions per week. Blood samples were gathered after 8 hours of fasting and 48 hours before and after the last training program. HPLC method was used to measure serum ceramide levels. The results showed There was no significant difference between the two resistance training protocols in serum ceramide (p = 0.71) and insulin sensitivity (p = 0.47). Two circular and traditional exercises with significant changes in lipid profile and significant increase in insulin sensitivity levels (p = 0.008), (p = 0.001) respectively. But decrease in ceramide (p = 0.001) was observed only in circular exercises. According to the results of the present study, that serum lipid and ceramide profile levels decreased during circular resistance training with increasing insulin sensitivity. And this decrease may indicate a decrease in metabolic risk factors.