Document Type : Research Paper
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Abstract
The purpose of this study was the effect of eighteen weeks’ endurance training and high-fat diet on body weight and amylin plasma levels of male Wistar rats. In order to, 24 young male Wistar rats were divided into three groups (n=8) includes base, control (C) and endurance training (ET) groups in age range 50 to 60 days and average weight 160±10 gr. The ET group rats run on treadmill without slope by 5 day/week, per session 15-65 min, 15-30 m/min velocity for eighteen weeks (incremental). Base group used for initial sampling and C group no train for eighteen weeks. High fat diet was included 60% fat, 24% CHO and 16% protein available two groups; C and ET. Blood sampling was conducted after latest training session with 12h fasting. Data were analyzed using the One-Way ANOVA and Tukey Post-Hoc tests (P<0.05). Data showed that the amylin levels was significantly higher in ET than base groups (P<0.0001). The amylin levels were significantly lower in training than control (no train) group (P<0.0001) and this value was no difference significant in ET than base group (P=0.47). On based of the Lee’s index the body weight was in the area of obesity in control group. The rats' body weight was significantly lower in ET than control group (P<0.0001). Therefore, ingestion of chronic high fat diet leads to the obesity and hyperamylinemia and adding a program of endurance training into this diet prevents with this condition.
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2. Mack C, Hoyt J, Moore C, Jodka C, Sams-Dodd F. Sustained reduction in food intake and body weight in high-fat-fed rats during 28-day amylin infusion. Diabetes. 2003; 52 (suppl. 1), A389.
3. Baran K, Preston E, Wilks D, Cooney G J, Kraegen E W, Sainsbury A. Chronic central melanocortin-4 receptor antagonism and central neuropeptide- Y infusion in rats produce increased adiposity by divergent pathways. Diabetes. 2002 ; 51 (1): 152–8.
4. Lutz T A. The interaction of amylin with other hormones in the control of eating. Diabetes Obes Metab. 2013; 15(2): 99-111.
5. Eiden S, Daniel C, Steinbrueck A, Schmidt I, Simon E. Salmon calcitonin–a potent inhibitor of food intake in states of impaired leptin signaling in laboratory rodents. J Physiol. 2002; 15; 541(pt3): 1041–8.
6. Rushing PA, Hagan M M, Seeley R J, Lutz T A, Woods S C. Amylin: A novel action in the brain to reduce body weight. Endocrinology. 2000; 141(2): 850–3.
7. Roth G D, Trevaskis J L, Wilson J, Lei C, Athanacio J, Mack C, et al. Ant obesity effects of the b-cell hormone amylin in combination with phentermine or sibutramine in diet-induced obese rats. International Journal of Obesity. 2008; 32 (8): 1201–10.
8. Lutz T A. Effects of amylin on eating and adiposity. Handb Exp Pharmacol. 2012; (209): 231-50.
9. Arnelo U, Reidelberger R, Adrian T E, Larsson J, Permert J. Sufficiency of postprandial plasma levels of islet amyloid polypeptide for suppression of feeding in rats. Am J Physiol Regul Integr Comp Physiol.1998; 275: 1537–42.
10. Guidobono F, Coluzzi M, Pagani F, Pecile A, Netti C. Amylin given by central and peripheral routes inhibits acid gastric secretion. Peptides. 1994;15 (4): 699–702.
11. Rushing P A, Hagan M M, Seeley R J, Lutz T A, Woods S C. Amylin: A novel action in the brain to reduce body weight. Endocrinology.2000;141(2): 850–3.
12. Reinehr T, de Sousa G, Niklowitz P, Roth C L. Amylin and its relation to insulin and lipids in obese children before and after weight loss. Obesity (Silver Spring). 2007; 15(8): 2006-11.
13. Reda T K, Geliebter A, Pi-Sunyer F X. Amylin, food intake, and obesity. Obes Res. 2002; 10 (10): 1087–91.
14. Young A, Denaro M. Roles of amylin in diabetes and in regulation of nutrient load. Nutrition. 1998; 14(6): 524–7.
15. Smith D, Mamo J C. Islet amyloid polypeptide (amylin) modulates chylomicron metabolism in rats. Clin Exp Pharmacol Physiol.2000; 27(5-6): 345–51.
16. Mather KJ, Paradisi G, Leaming R, Hook G, Steinberg HO, Fineberg N, et al. Role of amylin in insulin secretion and action in humans: antagonist studies across the spectrum of insulin sensitivity. Diabetes Metab Res Rev. 2002; 18(2): 118 –26.
17. Roberts C K, Izadpanah A, Angadi S S, Barnard R J. Effects of an intensive short-term diet and exercise intervention: Comparison between normal-weight and obese children. Am J Physiol Regul Integr Comp Physiol. 2013; 305(5): 552-7.
18. Croymans D M, Paparisto E, Lee M M, Brandt N, Le B K, Lohan D, et al. Resistance training improves indices of muscle insulin sensitivity and β-cell function in overweight/obese, sedentary young men. J Appl Physiol. 2013;115(9): 1245-53
19. Boyle C N, Rossier M M, Lutz T A. Influence of high-fat feeding, diet-induced obesity, and hyperamylinemia on the sensitivity to acute amylin. Physiol Behav. 2011;104(1): 20-8.
20. Roth J D. Amylin and the regulation of appetite and adiposity: Recent advances in receptor signaling, neurobiology and pharmacology. Curr Opin Endocrinol Diabetes Obes. 2013; 20(1): 8-13.
21. Miegueu P, St-Pierre D H, Munkonda M N, Lapointe M, Cianflone K. Amylin stimulates fatty acid esterification in 3T3-L1 adipocytes. Mol Cell Endocrinol. 2013; 366(1): 99-107.
22. Trevaskis J L, Coffey T, Cole R, Lei C, Wittmer C, Walsh B, et al. Amylin-mediated restoration of leptin responsiveness in diet-induced obesity: Magnitude and mechanisms. Endocrinology. 2008; 149(11): 5679-87.
23. Mack C, Wilson J, Athanacio J, Reynolds J, Laugero K, Guss S, et al. Pharmacological actions of the peptide hormone amylin in the long-term regulation of food intake, food preference, and body weight. Am J Physiol Regul Integr Comp Physiol. 2007; 293(5): 1855–63.
24. Trevaskis J L, Coffey T, Cole R, Lei C, Wittmer C, Walsh B, et al. Amylin-mediated restoration of leptin responsiveness in diet-induced obesity: Magnitude and mechanisms. Endocrinology. 2008; 149(11): 5679-87.
25. Young A, Denaro M. Roles of amylin in diabetes and in regulation of nutrient load. Nutrition. 1998; 14(6): 524–7.
26. Boyle C N, Christina N, Mélanie M. Rossier, Thomas A. Lutz. Influence of high-fat feeding, diet-induced obesity, and hyperamylinemia on the sensitivity to acute amylin. Physiology & Behavior. 2011;104(1): 20–28.
27. Lee M O. Determination of the surface area of the white rat with its application to the expression of metabolic results. Am J Physio. 1929; 89: 24–33.
28. El-Rasheidy O F, Amin D A, Ahmed H A, El Masry H, Montaser Z M. Amylin level and gastric emptying in obese children: Before and after weight loss. J Egypt Soc Parasitol. 2012; 42(2): 431-42.
29. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Reviews. 2010; 23(2): 270–99.
30. Ye JM, Lim-Fraser M, Cooney GJ, Cooper GJ, Iglesias MA, Watson DG, et al. Evidence that amylin stimulates lipolysis in vivo: A possible mediator of induced insulin resistance. Am J Physiol Endocrinol Metab.2001; 280(4): 562–9.
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