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The Effects of Vitamin D and Continuous Training on Apoptosis Markers in Aorta Tissue of Rats Exposed to Hydrogen Peroxide (H2O2)

Document Type : Research Paper

Authors

1 Ph.D. Student of Sport Physiology, Islamic Azad University, Tehran Branch

2 Associate Professor of Sport Physiology, Islamic Azad University Tehran Branch

3 Associate Professor of Sport Physiology, Islamic Azad University, Sari Branch

Abstract

The purpose of this study was to investigate the effect of vitamin D and continuous training on apoptotic markers of aorta tissue in poisoned water with oxygen. Thirty-six mature male Wistar rats (weighed on 220 ± 20gr, aged 8-10 weeks) were randomly assigned into 6 groups (n = 6) as follows; group 1; control group, group 2; received DMSO (Sham), group 3; Hydrogen peroxide, group 4; intraperitoneal injection of H2O2 + vitamin D, group 5; intraperitoneal injection of H2O2 + exercise, group 6; H2O2 + vitamin D + exercise. The animals were knocked out and the aortic tissue was isolated and the amounts of Caspase 3, Bax and Bcl2 proteins were measured by ELISA method. The data were analyzed by One-way ANOVA and P < 0.05 was considered as significant. Our results showed that the group which received only H2O2T significantly increased Bax, Caspase 3, and Bax/Bcl2 ratio compared to other groups (P < 0.05). also, we found that Vitamin D supplements, continuous training and combination of both reduced Bax, Caspase 3, and Bax/Bcl2 ratio, and increased Bcl2 levels compared to group which received only H2O2 (P < 0.05). According to the results, continue exercise with vitamin D supplementation decreased aorta by reducing Bax/Bcl2 ration and probably protects aorta tissue from damaging.

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References
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Sport Physiology
Volume 10, Issue 40
Winter 2019
January 2019
Pages 163-178
Files
History
  • Receive Date: 15 February 2019
  • Revise Date: 26 May 2019
  • Accept Date: 01 June 2019
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