نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکز

2 استاد فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکز

3 دانشیار علوم شناختی دانشگاه علوم‌پزشکی، دانشگاه آزاد اسلامی واحد پزشکی، تهران

چکیده

هدف از پژوهش حاضر، بررسی تأثیر یک دوره تمرین تناوبی شدید و مکمل‌سازی روغن بذر کتان بر درد القا‌شده در موش‌های صحرایی نر بود. بدین‌منظور، 40 سر موش صحرایی بالغاز نژاد ویستار به‌عنوان نمونۀ آماری انتخاب شده و به‌صورت تصادفی به هشت گروه (پنج سر در هر گروه) کنترل ـ سالین، تمرین ـ سالین، عصارۀ دوز 100، عصارۀ دوز 200 ، عصارۀ دوز 300، تمرین ـ عصارۀ دوز 100، تمرین ـ عصارۀ دوز 200 و تمرین ـ عصارۀ دوز 300 میلی‌گرم بر کیلوگرمتقسیم گردیدند. گروه‌های تمرینی با 90 تا 95 درصد حداکثر اکسیژن مصرفی به تمرین اینتروال روی نوارگردان مخصوص جوندگان پرداختند (10 هفته، پنج جلسه در هفته) و گروه‌های مکمل، روغن بذر کتان را دریافت نمودند. ارزیابی آستانۀ احساس درد توسط آزمون صفحۀ داغ با دمای 5/0±55 درجۀ سانتی‌گراد صورت گرفت. برای بررسی تفاوت بین گروه‌ها نیز از آزمون آنالیز واریانس دوطرفه استفاده شد و سطح معناداری کمتر از (05/0) لحاظ گردید. نتایج نشان می‌دهد که استفاده از مکمل باعث کاهش معنادار احساس درد شده است (P=0.004) که البته این کاهش در گروه عصاره دوز 300 به‌شکل معناداری بالاتر از گروه عصاره دوز 100 است (P=0.012). همچنین، احساس درد در گروه‌های تعاملی تمرین مکمل به‌طور معناداری کمتر از گروه‌های تمرین و کنترل ـ سالین می‌باشد (P<0.05). احتمالاً مصرف مکمل به‌دلیل وجود ویتامین‌ها و روغن‌های غیراشباع مفید می‌تواند احساس درد ناشی از تمرین شدید را تعدیل کند؛ بنابراین، استفاده از روغن بذر کتان جهت کاهش درد ناشی از فعالیت بدنی سنگین توصیه می‌گردد. 

کلیدواژه‌ها

موضوعات

1. Terman G W, Shavit Y, Lewis J W, Cannon J T, Liebeskind J C. Intrinsic mechanisms of pain inhibition: Activation by stress. Science. 1984; 226(4680): 1270-7.
2. Dufton L M, Konik B, Colletti R, Stanger C, Boyer M, Morrow S, et al. Effects of stress on pain threshold and tolerance in children with recurrent abdominal pain. Pain. 2008; 136(1-2): 38-43.
3. Vachon-Presseau E, Martel M O, Roy M, Caron E, Albouy G, Marin M F, et al. Acute stress contributes to individual differences in pain and pain-related brain activity in healthy and chronic pain patients. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience. 2013; 33(16): 6826-33.
4. Vedolin G M, Lobato V V, Conti P C, Lauris J R. The impact of stress and anxiety on the pressure pain threshold of myofascial pain patients. J Oral Rehabil. 2009; 36(5): 313-21.
5. Ashkinazi I, Vershinina E A. Pain sensitivity in chronic psychoemotional stress in humans. Neurosci Behav Physiol. 1999; 29(3): 333-7.
6. Beecher H K. Pain in men wounded in battle. Bulletin of the US Army Medical Department. United States Army Medical Department. 1946; 5: 445-54.
7. Beecher H K. Relationship of significance of wound to pain experienced. Journal of the American Medical Association. 1956; 161(17): 1609-13.
8. Tierney G, Carmody J, Jamieson D. Stress analgesia: The opioid analgesia of long swims suppresses the non-opioid analgesia induced by short swims in mice. Pain. 1991; 46(1): 89-95.
9. Sheahan T D, Copits B A, Golden J P, Gereau R W T. Voluntary exercise training: Analysis of Mice in uninjured, inflammatory, and nerve-injured pain states. PLoS One. 2015; 10(7): 133-91.
10. Shokraviyan M, Miladi-Gorji H, Vaezi Gh. Voluntary and forced exercises prevent the development of tolerance to analgesic effects of morphine in rats. Iran J Basic Med Sci. 2014; 17(4): 271-7. (In Persian).
11. Koltyn K F. Analgesia following exercise: A review. Sports Medicine. 2000; 29(2): 85-98.
12. Morgan D, Mitzelfelt J D, Koerper L M, Carter C S. Effects of morphine on thermal sensitivity in adult and aged rats. The Journals of Gerontology Series A, Biological Sciences and Medical Sciences. 2012; 67(7): 705-13.
13. Motaghinejad M, Ghaleni M A, Motaghinejad O. Preventive effects of forced exercise against alcohol-induced physical dependency and reduction of pain perception threshold. International Journal of Preventive Medicine. 2014; 5(10): 1299-307. (In Persian).
14. Rossi D M, Valenti V E, Navega M T. Exercise training attenuates acute hyperalgesia in streptozotocin-induced diabetic female rats. Clinics. 2011; 66(9): 1615-9.
15. Kolodny A L. Side-effects produced by alcohol in a patient receiving furazolidone. Md State Med J. 1962; 11: 248.
16. Jacobson L, Chabal C, Brody M C. A dose-response study of intrathecal morphine: Efficacy, duration, optimal dose, and side effects. Anesth Analg. 1988; 67(11): 1082-8.
17. Arita H, Hayashida M, Shu H, Xu H, Sekiyama H, Hanaoka K. Application of herb medicine in pain clinic-focusing on the basic research of Aconiti tuber. Masui the Japanese Journal of Anesthesiology. 2007; 56 Suppl: 199-211.
18. Bao Y M, Luo F, Wei J H. Forty cases of chronic prostatitis/ chronic pelvic pain syndrome treated by acupuncture and crude herb moxibustion. Zhongguo Zhen Jiu: Chinese Acupuncture & Moxibustion. 2011; 31(6): 571-2.
19. Fan H, Li T F, Gong N, Wang Y X. Shanzhiside methylester, the principle effective iridoid glycoside from the analgesic herb Lamiophlomis rotata, reduces neuropathic pain by stimulating spinal microglial beta-endorphin expression. Neuropharmacology. 2016; 101: 98-109.
20. Kakilashvili B, Zurabashvili D Z, Turabelidze D G, Shanidze L A, Parulava G K. The fatty acid composition of ordinary flax seed oil (Linum usitatissimum L.) cultivated in Georgia and its byological activity. Georgian Med News. 2014; (227): 86-8.
21. Kargar R, Forouzanfar M, Ghalamkari G, Nasr Esfahani M H. Dietary flax seed oil and/or vitamin E improve sperm parameters of cloned goats following freezing-thawing. Cryobiology. 2016; 74: 110-4
22. Tuluce Y, Ozkol H, Koyuncu I. Photoprotective effect of flax seed oil (Linum usitatissimum L.) against ultraviolet C-induced apoptosis and oxidative stress in rats. Toxicology and Industrial Health. 2012; 28(2): 99-107.
23. Williams D, Verghese M, Walker L T, Boateng J, Shackelford L, Chawan C B. Flax seed oil and flax seed meal reduce the formation of aberrant crypt foci (ACF) in azoxymethane-induced colon cancer in Fisher 344 male rats. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association. 2007; 45(1): 153-9.
24. Ghanbari-Niaki A, Rahmati-Ahmadabad S. Effects of a fixed-intensity of endurance training and pistacia atlantica supplementation on ATP-binding cassette G4 expression. Chin Med. 2013; 8(1): 23.
25. Bayati M, Gharakhanlou R, Farzad B. Adaptations of physiological performance following high-intensity interval training. Sport Physiology. 2015; 7(26): 15-32. (In Persian).
26. Shafiee A, Kordi M, Gaeini A, Soleimani M, Nekouei A, Hadidi V. The effect of eight week of high intensity interval training on expression of Mir-210 and Ephrin A3 Mrna in soleus muscle healthy male rats. Arak University of Medical Sciences Journal. 2014; 17(3): 26-34. (In Persian).
27. Hoydal M A, Wisloff U, Kemi O J, Ellingsen O. Running speed and maximal Oxygen uptake in Rats and Mice: Practical implications for exercise training. European Journal of Cardiovascular Prevention and Rehabilitation: Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology. 2007; 14(6): 753-60.
28. Bannon A W, Malmberg A B. Current Protocols in Neuroscience. 40thed. Hoboken: John Wiley and Sons, Inc ;2007. p. 209-30.
29. Bodnar R J, Kelly D D, Spiaggia A, Ehrenberg C, Glusman M. Dose-dependent reductions by naloxone of analgesia induced by cold-water stress. Pharmacology, Biochemistry, and Behavior. 1978; 8(6): 667-72.
30. Christie M J, Chesher G B, Bird K D. The correlation between swim-stress induced antinociception and [3H] leu-enkephalin binding to brain homogenates in Mice. Pharmacology, Biochemistry, and Behavior. 1981; 15(6): 853-7.
31. Yao T, Andersson S, Thoren P. Long-lasting cardiovascular depressor response following sciatic stimulation in spontaneously hypertensive rats. Evidence for the involvement of central endorphin and serotonin systems. Brain Research. 1982; 244(2): 295-303.
32. Geisser M E, Wang W, Smuck M, Koch L G, Britton S L, Lydic R. Nociception before and after exercise in rats bred for high and low aerobic capacity. Neuroscience Letters. 2008; 443(1): 37-40.
33. Devaraj S, Jialal I. Alpha tocopherol supplementation decreases serum C-reactive protein and monocyte interleukin-6 levels in normal volunteers and type 2 diabetic patients. Free Radical Biology & Medicine. 2000; 29(8): 790-2.
34. Upritchard J E, Sutherland W H, Mann J I. Effect of supplementation with tomato juice, vitamin E, and vitamin C on LDL oxidation and products of inflammatory activity in type 2 diabetes. Diabetes Care. 2000; 23(6): 733-8.
35. Saremi A, Changizi Ashtiyani S, Kalantari A. The combination of vitamin E supplementation and intensive exercise on testicular oxidative stress and spermatogenesis in male rats. Sport Physiology. 2014; 6(23): 43-54. (In Persian)
36. Goyal A, Sharma V, Sihag M K, Singh A K, Arora S, Sabikhi L. Fortification of dahi (Indian yoghurt) with omega-3 fatty acids using microencapsulated flaxseed oil microcapsules. J Food Sci Technol. 2016; 53(5): 2422-33.
37. Goyal A, Sharma V, Upadhyay N, Singh A K, Arora S, Lal D, et al. Development of stable flaxseed oil emulsions as a potential delivery system of omega-3 fatty acids. J Food Sci Technol. 2015; 52(7): 4256-65.
38. Costantino D, Guaraldi C, Costantino M, Bounous V E. Use of alpha-lipoic acid and omega-3 in postpartum pain treatment. Minerva Ginecol. 2015; 67(5): 465-73.
39. Maroon J C, Bost J W. Omega-3 fatty acids (fish oil) as an anti-inflammatory: An alternative to nonsteroidal anti-inflammatory drugs for discogenic pain. Surg Neurol. 2006; 65(4): 326-31.
40. Bolboli L, Ghafari G, Rajabi A. Effect of omega-3 consumption and participate in aerobic exercise on sICAM-1 and pro-inflammatory cytokines in obese elderly women. Sport Physiology. 2014; 6(21): 79-94. (In Persian).
41. Shaabani Y A R, Taleban R A, Jamshidi F A, Akhlaghi A A, Alavi M A. Comparison of the effect of Omega-3 fatty acid supplementation with and without vitamin E in patients with rheumatoid arthritis. Iranian Journal of Nutrition Sciences & Amp; Food Technology. 2007; 2(2): 57-69. (In Persian).