افزایش ماکروفاژهای آلوئولی و سورفکتانت ریوی رت های نر جوان پس از شش هفته تمرین تناوبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد فیزیولوژی ورزشی، دانشگاه مازندران

2 کارشناسی ارشد فیزیولوژی ورزشی، دانشگاه مازندران

3 استادیار بافت شناسی، دانشگاه تبریز

4 دانشیار بیوشیمی، پژوهشکده علوم غدد و متابولیسم، دانشگاه علوم پزشکی شهید بهشتی

چکیده

هدف این پژوهش بررسی تغییرات ماکروفاژ آلوئولی و سطوح پروتئین سورفکتانت ریوی در پی شش هفته تمرین تناوبی در رت های نر جوان بود. 15 سر موش صحرائی نر نژاد ویستار سه هفته ای با میانگین وزنی 8± 96 گرم به طور تصادفی به گروه­های پایه، کنترل و تمرین تقسیم شدند. پس از دو هفته آشنایی با محیط آزمایشگاه و نوارگردان، شش هفته تمرین تناوبی را اجرا کردند. آزمودنی ها شش جلسه در هفته، هر جلسه 30 دقیقه تمرین تناوبی را اجرا کردند. یک دقیقه فعالیت شدید با دو دقیقه استراحت فعال در 10 تکرار اجرا شد. سرعت دویدن آزمودنی ها در زمان استراحت، نصف سرعت تمرین در نظر گرفته شد. اندازه گیری سطوح پروتئین سورفکتانت A با روش الایزا و درصد جمعیت ماکروفاژهای آلوئولی با استفاده از روش استریولوژی بافت ریه صورت گرفت. تجزیه و تحلیل با استفاده از آنالیز واریانس یک طرفه و آزمون LSD در سطح  P≤0.05 انجام شد. شش هفته تمرین تناوبی موجب افزایش معنی دار در سطوح سورفکتانت A نسبت به گروه پایه و کنترل به ترتیب با 33.2 و 24.2 درصد شد (0.001P≤). همچنین درصد جمعیت ماکروفاژهای آلوئولی نسبت به گروه پایه و کنترل به طور معنی داری (0.001P≤) افزایش یافت (به ترتیب 660 و 11 برابر). با تأیید اثر تعاملی بین ماکروفاژهای آلوئولی و سورفکتانت A در مواجهه با فعالیت ورزشی، و نقش برجسته تر ماکروفاژهای آلوئولی نسبت به سورفکتانت A ، نقش تنظیمی سورفکتانت A در کنترل التهاب احتمالی ناشی از ورزش و حمایت از ماکروفاژهای آلوئولی مورد انتظار است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Increment of Alveolar Macrophages and Pulmonary Surfactant of Young Male Rats after Six Weeks Interval Training

نویسندگان [English]

  • Shadmehr Mirdar 1
  • Froogh Naiestany 2
  • Gholamreza Hamidian 3
  • Mahdi Hedayati 4
1 Associate Professor of Sport Physiology, University of Mazandaran
2 M.Sc. Student in Sport Physiology, University of Mazandaran
3 Assistant Professor of Histology, University of Tabriz
4 Associate Professor of Biochemistry, Shaheed Beheshti University of Medical Sciences
چکیده [English]

This study aims to investigate changes of Alveolar macrophage and protein levels of pulmonary surfactant due to the six weeks of interval training on young male rats. Fifteen male Wistar rats strain in age by 3-weeks with average weight of 68 ± 9 g, were randomly divided into three experimental of basic, control and training groups. Progressive interval training was performed for six weeks after 2 weeks of familiarity with the lab and the treadmill. Exercises were performed for six days a week, 30-min during each interval training session. Training was carried out 1-min of arduous exercise with 2-min active rest interval in ten repetitions. Half of the training speed was considered for treadmill running speeds of trained rates in period of rest time. Surfactant protein A (SP-A) levels was measured by ELISA method and also Alveolar macrophages population percentages were measured by using Stereology of lung tissue method before and after the training program. Data were analyzed by ANOVA and LSD test at P≤0.05. Six weeks of progressive interval training significantly increased SP-A (P≤0.001) compared to the basic and control groups (32.2 and 24.2 percent respectively). Moreover, these trains significantly increased Alveolar macrophage population percentage than the basic and control groups at P≤0.001 (660 and 11- fold respectively).Physical activities confirmed the interaction effect of Alveolar macrophage and SP-A while, Alveolar macrophage had a more prominent role than the SP-A. It is expected that SP-A had a regulatory role in controlling inflammation and protection of the alveolar macrophage.

کلیدواژه‌ها [English]

  • Alveolar Macrophage
  • Interval Training
  • Surfactant
  • Lung Inflammation
  • Innate Immune
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