تأثیر تمرین تناوبی هوازی با شدت بالا بر آنژیوژنز بافت چربی در موش‌های صحرایی تغذیه‌شده با رژیم غذایی پرچرب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری فیزیولوژی ورزشی، دانشگاه مازندران

2 دانشیار فیزیولوژی ورزشی، دانشگاه مازندران

3 استادیار علوم پایه، دانشگاه تبریز

چکیده

هدف پژوهش حاضر، بررسی تأثیر تمرین تناوبی با شدت بالا بر مورفولوژی و آنژیوژنز بافت چربی احشایی، در موش‌های صحرایی تغذیه‌‌شده با غذای پرچرب بود. تعداد 20 سر رت نر از نژاد ویستار با دامنة سنی چهار تا شش هفته و میانگین وزنی 73/3 ± 2/176 گرم، براساس همگن‌سازی وزنی ابتدا به دو گروه تغذیه با غذای نرمال و غذای پرچرب (10 سر در هر گروه) تقسیم شدند. پس از 10 هفته، دوباره هریک ازگروه‌ها به دو گروه مجزا (کنترل و تمرین) تقسیم شدند (پنج سر در هر گروه). گروه‌های تمرینی برای 10 هفته (پنج روز در هفته) تمرین تناوبی هوازی با شدت بالا را روی نوار گردان انجام دادند. حجم سلول چربی با روش استریولوژی و همچنین، چگالی مویرگی بافت چربی با روش ایمنوفلورسانس ارزیابی شدند. نتایج نشان داد که رژیم غذایی پرچرب منجر به افزایش معنادار وزن بدن (P < 0.001)، وزن بافت چربی(P < 0.001متوسط حجم سلول‌های چربی (P < 0.001گلوکز پلاسمایی (P < 0.001) و کاهش چگالی مویرگی    (P < 0.001) نسبت به رژیم غذایی نرمال می‌شود. همچنین، تمرین تناوبی با شدت بالا موجب کاهش معنا‌دار وزن بدن (P < 0.001وزن بافت چربی (P < 0.001)، حجم متوسط سلول‌های چربی (P < 0.001)، گلوکز پلاسما (P = 0.01)، انسولین (P = 0.003) و شاخص مقاومت انسولینی (P = 0.002) نسبت به رژیم غذایی به‌تنهایی می‌شود. به‌علاوه، آنژیوژنز بافت چربی در گروه تمرین + غذای پرچرب در مقایسه با گروه‌غذای پرچرب، به‌طور معنا‌داری بیشـتـر است (P < 0.001).نتایج پژوهش حاضر نشان داد که تمرین تناوبی با شدت بالا موجب کاهش وزن بدن و وزن بافت چربی، ازطریق افزایش آنژیوژنز و کاهش حجم سلول‌های چربی می‌شود که با بهبود هومئوستاز گلوکز همراه است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effects of High-Intensity Intermittent Aerobic Training on Adipose Tissue Angiogenesis in Rats Fed a High Fat Diet

نویسندگان [English]

  • Sarkawt Kolahdouzi 1
  • Elahe Talebi Garakani 2
  • Gholamreza Hamidian 3
  • Alireza Safarzade 2
1 Ph.D. Exercise Physiology, University of Mazandaran
2 Associate Professor of Exercise Physiology, University of Mazandaran
3 Assistant professor of Baisc Science, University of Tabriz
چکیده [English]

The purpose of this study was to evaluate of high intensity intermittent training on angiogenesis and morphological changes of visceral adipose tissue in high fat fed male wistar rats. Twenty male wistar rats (4-6 weeks age, and body weight: Mean±SE: 176.2±3.73) were homogenously divided into two groups of high fat diet (n: 10) and standard diet regimens (n: 10). After 10 weeks, each group divided into two groups (training and control; n: 5 in each group); the training group completed 10 weeks high-intensity intermittent aerobic training on a motorized treadmill. Mean adipocyte size and angiogenesis of mesenteric fat were analyzed by Stereology and Immunofluorescence methods respectively. The results showed that high fat diet regimen causes elevation in body weight (P<0.001), fat pad weight (P<0.001), mean adipocyte size (P<0.001), plasma glucose (P<0.001), and reduction in angiogenesis (P<0.001) compared to the standard diet regimen. The high intensity intermittent training caused reduction in body weight (P<0.001), fat pad weight (P<0.001), mean adipocyte size (P<0.001), plasma glucose (P=0.01), plasma insulin (P=0.003) and insulin resistance index (P=0.002) compared to the diet regimen alone (P<0.05). Moreover, in the training+high fat diet group angiogenesis increased compared to the high fat regimen control group (P<0.001). The results indicated that high intensity intermittent aerobic training induces reduction in body weight and fat weight by elevation in angiogenesis and reduction in mean adipocyte size during high fat feeding that is accompanied with improvement of blood glucose hemostasis.

کلیدواژه‌ها [English]

  • Adipose Tissue
  • angiogenesis
  • High Intensity Exercise Training
  • High Fat Diet
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