نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
2 استاد فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
3 استاد جنین شناسی، گروه زیست‌شناسی، پژوهشکده زیست‌فناوری پژوهشگاه رویان، اصفهان، ایران
چکیده
شرایط استرس‌زای پیش دیابت، به مرحله قبل از شکل‌گیری بیماری دیابت نوع ۲ گفته می‌شود، که میزان قند خون بیشتر از حد طبیعی و کمتر از معیارهای تشخیصی دیابت نوع ۲ است. بنابراین هدف پژوهش حاضر، بررسی تأثیر تمرین استقامتی بر ذخایر گلیکوژن عضلانی موش‌های پیش دیابتی شده می باشد. بر این اساس تعداد 15 سر موش نر نژاد C57BL/6 به‌طور تصادفی به دو گروه تقسیم شدند: موش‌های تغذیه‌شده با رژیم غذایی طبیعی (ND ، 5 عدد) و رژیم غذایی پرچرب (HFD ، تعداد 10 عدد). موش‌ها به مدت ۱۲ هفته تغذیه شدند. پس از تشخیص القای پیش دیابت توسط آزمون های تشخیصی در گروه HFD ، این گروه به دو زیرگروه تقسیم شدند: موش‌های پیش دیابت شده (PD) و موش‌های پیش دیابت شده به همراه تمرین استقامتی (PD-Ex) نتایج کاهش معناداری از سطح گلوکز خون و مقاومت انسولینی را در گروه PD-Ex نسبت به PD نشان داد. تکنیک PAS تغییرات کاهش قابل‌مشاهده ای از ذخایر گلیکوژن در HFD نسبت به گروه ND نشان داد درحالیکه تمرین استقامتی باعث افزایش تجمع گلیکوژن در عضله اسکلتی موش‌ها شد. سطح بیان ژن‌های نسبی دخیل در ذخایر کیلگوژن عضله همچونPI3K, AKT, GSK3,GYS1، و ژن LRRC8A تغییرات معناداری (05/0>p) بین گروه ND در مقایسه با گروه HFD نشان داد، که نشان‌دهنده اختلال مسیر سیگنالینگ ذخایر گلیکوژن می‌باشد.تمرین استقامتی (PD-Ex) منجر به بهبود قابل‌ملاحظه‌ای از سطح بیان ژن GSK3 شد. به طور کلی به نظر می‌رسد انجام تمرین استقامتی، تأثیرات منفی شرایط پیش دیابتی ناشی از مصرف رژیم غذایی پرچرب را بهبود می بخشد.
کلیدواژه‌ها
- Deedwania PC, Fonseca VA. Diabetes, prediabetes, and cardiovascular risk: shifting the paradigm. The American journal of medicine. 2005;118(9):939-47.
- Abbasi B, Molavi N, Tavalaee M, Abbasi H, Nasr-Esfahani MH. Alpha-lipoic acid improves sperm motility in infertile men after varicocelectomy: a triple-blind randomized controlled trial. Reproductive BioMedicine Online. 2020;41(6):1084-91.
- Tabák AG, Herder C, Rathmann W, Brunner EJ, Kivimäki M. Prediabetes: a high-risk state for diabetes development. The Lancet. 2012;379(9833):2279-90.
- Li Y-p, Xiao J, Liang X, Pei Y, Han X-f, Li C-x, et al. DPP-4 inhibition resembles exercise in preventing type 2 diabetes development by inhibiting hepatic protein kinase Cε expression in a mouse model of hyperinsulinemia. Journal of International Medical Research. 2020;48(6):0300060520934635.
- Solayman M, Ali Y, Alam F, Islam A, Alam N, Khalil I, et al. Polyphenols: potential future arsenals in the treatment of diabetes. Current pharmaceutical design. 2016;22(5):549-65.
- Ng JM, Azuma K, Kelley C, Pencek R, Radikova Z, Laymon C, et al. PET imaging reveals distinctive roles for different regional adipose tissue depots in systemic glucose metabolism in nonobese humans. American Journal of Physiology-Endocrinology and Metabolism. 2012;303(9):E1134-E41.
- Catherine M, Kenneth C. Prediabetes: A Worldwide Epidemic. Endocrinol Metab Clin N Am. 2016;45(4,751-764).
- Kuai M, Li Y, Sun X, Ma Z, Lin C, Jing Y, et al. A novel formula Sang-Tong-Jian improves glycometabolism and ameliorates insulin resistance by activating PI3K/AKT pathway in type 2 diabetic KKAy mice. Biomedicine & Pharmacotherapy. 2016;84:1585-94.
- Højlund K. Metabolism and insulin signaling in common metabolic disorders and inherited insulin resistance. Danish Medical Journal. 2014. 61(7):B4890
- Akmali A, Saghebjoo M. High-intensity interval training with long duration intervals is more effective than short duration intervals for improving glycolytic capacity in the rats’ gastrocnemius muscle. Hormone molecular biology and clinical investigation. 2020;41(2).
- Orho-Melander M, Almgren P, Kanninen T, Forsblom C, Groop LC. A paired-sibling analysis of the XbaI polymorphism in the muscle glycogen synthase gene. Diabetologia. 1999;42(9):1138-45.
- Strange K, Yamada T, Denton JS. A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel. Journal of General Physiology. 2019;151(2):100-17.
- Osei-Owusu J, Yang J, del Carmen Vitery M, Qiu Z. Molecular biology and physiology of volume-regulated anion channel (VRAC). Current topics in membranes. 2018;81:177-203.
- Kumar A, Xie L, Ta CM, Hinton AO, Gunasekar SK, Minerath RA, et al. SWELL1 regulates skeletal muscle cell size, intracellular signaling, adiposity and glucose metabolism. Elife. 2020;9:e58941.
- Haidari F, Samadi M, Mohammadshahi M, Jalali MT, Engali KA. Energy restriction combined with green coffee bean extract affects serum adipocytokines and the body composition in obese women. Asia Pacific journal of clinical nutrition. 2017;26(6):1048-54.
- Stump CS, Henriksen EJ, Wei Y, Sowers JR. The metabolic syndrome: role of skeletal muscle metabolism. Annals of medicine. 2006;38(6):389-402.
- Roberts CK, Won D, Pruthi S, Barnard RJ. Effect of a diet and exercise intervention on oxidative stress, inflammation and monocyte adhesion in diabetic men. Diabetes research and clinical practice. 2006;73(3):249-59.
- Laboratory J. Phenotype Information For Diet-Induced Obese C57BL/6J (380050). . Available at: https://wwwjaxorg/jax-mice-and-services/strain-data-sheet-pages/phenotype-information-380050.
- Dougherty JP, Springer DA, Gershengorn MC. The treadmill fatigue test: a simple, high-throughput assay of fatigue-like behavior for the mouse. JoVE (Journal of Visualized Experiments). 2016(111):e54052.
- Powers SK, Criswell D, Lawler J, Martin D, Lieu F-K, Ji LL, et al. Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. American Journal of Physiology-Heart and Circulatory Physiology. 1993;265(6):H2094-H8.
- Powers SK, Howley ET, Quindry J. Exercise physiology: Theory and application to fitness and performance: McGraw-Hill New York, NY; 2007.
- Kazeminasab F, Marandi SM, Ghaedi K, Safaeinejad Z, Esfarjani F, Nasr-Esfahani MH. A comparative study on the effects of high-fat diet and endurance training on the PGC-1α-FNDC5/irisin pathway in obese and nonobese male C57BL/6 mice. Applied physiology, nutrition, and metabolism. 2018;43(7):651-62.
- Abdollahi M, Marandi SM, Ghaedi K, Safaeinejad Z, Kazeminasab F, Shirkhani S, et al. Insulin-Related Liver Pathways and the Therapeutic Effects of Aerobic Training, Green Coffee, and Chlorogenic Acid Supplementation in Prediabetic Mice. Oxidative Medicine and Cellular Longevity. 2022;2022.
- Riccardi G, Giacco R, Rivellese A. Dietary fat, insulin sensitivity and the metabolic syndrome. Clinical nutrition. 2004;23(4):447-56.
- Cheong JLK, Croft K, Henry P, Matthews V, Hodgson J, Ward N. Green coffee polyphenols do not attenuate features of the metabolic syndrome and improve endothelial function in mice fed a high fat diet. Archives of biochemistry and biophysics. 2014;559:46-52.
- Church T. Exercise in obesity, metabolic syndrome, and diabetes. Progress in cardiovascular diseases. 2011;53(6):412-8.
- Motahari-Tabari N, Shirvani MA, Shirzad-e-Ahoodashty M, Yousefi-Abdolmaleki E, Teimourzadeh M. The effect of 8 weeks aerobic exercise on insulin resistance in type 2 diabetes: a randomized clinical trial. Global journal of health science. 2015;7(1):115.
- Li T, Ruan D-G, Lin Z-M, Liu T-Y, Wang K, Xu X-Y, et al. Endurance Training Counteracts the High-Fat Diet-Induced Profiling Changes of ω-3 Polyunsaturated Fatty Acids in Skeletal Muscle of Middle-Aged Rats. Frontiers in physiology. 2019:971.
- Li T, Ruan D-G, Gao J-J, Wang H, Xu X-Y. Role of skeletal muscle fat ectopic deposition in insulin resistance induced by high-fat diet. Sheng li xue bao:[Acta Physiologica Sinica]. 2018;70(4):433-44.
- Touati S, Meziri F, Devaux S, Berthelot A, Touyz RM, Laurant P. Exercise reverses metabolic syndrome in high-fat diet-induced obese rats. Medicine and science in sports and exercise. 2011;43(3):398-407.
- Hermansen L, Hultman E, Saltin B. Muscle glycogen during prolonged severe exercise. Acta Physiologica Scandinavica. 1967;71(2‐3):129-39.
- Jensen J, Lai Y-C. Regulation of muscle glycogen synthase phosphorylation and kinetic properties by insulin, exercise, adrenaline and role in insulin resistance. Archives of physiology and biochemistry. 2009;115(1):13-21.
- Damsbo P, Vaag A, Hother-Nielsen O, Beck-Nielsen H. Reduced glycogen synthase activity in skeletal muscle from obese patients with and without type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia. 1991;34(4):239-45.
- Johnson A, Webster J, Sum C-F, Heseltine L, Argyraki M, Cooper B, et al. The impact of metformin therapy on hepatic glucose production and skeletal muscle glycogen synthase activity in overweight type II diabetic patients. Metabolism. 1993;42(9):1217-22.
- Shulman GI, Rothman DL, Jue T, Stein P, DeFronzo RA, Shulman RG. Quantitation of muscle glycogen synthesis in normal subjects and subjects with non-insulin-dependent diabetes by 13C nuclear magnetic resonance spectroscopy. New England Journal of Medicine. 1990;322(4):223-8.
- Kim Y-B, Nikoulina SE, Ciaraldi TP, Henry RR, Kahn BB. Normal insulin-dependent activation of Akt/protein kinase B, with diminished activation of phosphoinositide 3-kinase, in muscle in type 2 diabetes. The Journal of clinical investigation. 1999;104(6):733-41.
- Morino K, Petersen KF, Dufour S, Befroy D, Frattini J, Shatzkes N, et al. Reduced mitochondrial density and increased IRS-1 serine phosphorylation in muscle of insulin-resistant offspring of type 2 diabetic parents. The Journal of clinical investigation. 2005;115(12):3587-93.
- He J, Kelley DE. Muscle glycogen content in type 2 diabetes mellitus. American Journal of Physiology-Endocrinology and Metabolism. 2004;287(5):E1002-E7.
- Hickner R, Fisher J, Hansen P, Racette S, Mier C, Turner M, et al. Muscle glycogen accumulation after endurance exercise in trained and untrained individuals. Journal of Applied Physiology. 1997;83(3):897-903.
- Burgomaster KA, Hughes SC, Heigenhauser GJ, Bradwell SN, Gibala MJ. Six sessions of sprint interval training increases muscle oxidative potential and cycle endurance capacity in humans. Journal of applied physiology. 2005.
- Bergström J, Hultman E. Muscle glycogen synthesis after exercise: an enhancing factor localized to the muscle cells in man. Nature. 1966;210(5033):309-10.
- Houmard JA, Shaw CD, Hickey MS, Tanner CJ. Effect of short-term exercise training on insulin-stimulated PI 3-kinase activity in human skeletal muscle. American Journal of Physiology-Endocrinology And Metabolism. 1999;277(6):E1055-E60.
- Christ-Roberts CY, Pratipanawatr T, Pratipanawatr W, Berria R, Belfort R, Kashyap S, et al. Exercise training increases glycogen synthase activity and GLUT4 expression but not insulin signaling in overweight nondiabetic and type 2 diabetic subjects. Metabolism. 2004;53(9):1233-42.
- Frøsig C, Rose AJ, Treebak JT, Kiens B, Richter EA, Wojtaszewski JF. Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160. Diabetes. 2007;56(8):2093-102.
- Wadley GD, Konstantopoulos N, Macaulay L, Howlett KF, Garnham A, Hargreaves M, et al. Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training. Journal of Applied Physiology. 2007;102(4):1624-31.
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