تغییرات مسیر ژنی TSC2(RAGs)/mTOR /4E-BPs بافت کلیه رت های نر بدنبال یک دوره تمرین مقاومتی و مصرف مکمل اسپیرولینا پلانتسیس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی وتغذیه ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

2 استادیار، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران

3 دانشیار ، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه خلیج فارس، بوشهر، ایران.

4 دانشیار، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، ایران

چکیده

هدف: تاثیر تمرینات مقاومتی در کنار مکمل های گیاهی بر مسیر پیام رسان mTOR در بافت های غیرعضلانی کمتر بررسی شده است. هدف از تحقیق حاضر ارزیابی تاثیر یک دوره تمرین مقاومتی و مکمل اسپیرولینا بر مسیر ژنی TSC2(RAGs)/mTOR /4E-BPs بافت کلیه موش های صحرایی نر بود. روش: تعداد 32 موش صحرایی 3ماهه و با میانگین وزن 20±150 گرم به طور تصادفی به چهارگروه کنترل، تمرین مقاومتی، مکمل اسپیرولینا و تمرین+مکمل اسپیرولینا تقسیم شدند. گروه های مکمل هرروز 200 میلیگرم اسپیرولینا مصرف می کردند. پروتکل تمرین مقاومتی به مدت هشت هفته، هفته ای 3 روز و یکبار در روز انجام شد. میزان بیان متغییرهای وابسته تحقیق با روش Real Time_PCR اندازه گیری و با تحلیل واریانس دوراهه مورد تجزیه قرار گرفتند. یافته‌ها: افزایش معناداری در میزان بیان ژن 4E_PBs در گروه تعاملی مکمل اسپیرولینا و تمرین مشاهده شد (012/0 P=)، ژن TSC2 در هیچ کدام از گروهها نسبت به گروه کنترل افزایش معناداری نداشت. در فاکتور RAGs افزایش معناداری در هر سه گروه مکمل اسپیرولینا (001/0 P=)،گروه تمرین مقاومتی (001/0 P=) و گروه تعامل تمرین مقاومتی و مکمل (001/0 P=) مشاهده شد. همچنین افزایش معناداری در میزان بیان ژن mTOR در گروه مکمل اسپیرولینا (001/0 P=) و گروه تمرین مقاومتی (014/0 P=) مشاهده شد. نتیجه گیری: با توجه به اینکه که در گروه تعامل تمرین مقاومتی با مصرف مکمل اسپیرولینا افزایش معناداری در بیان ژن mTOR مشاهده نشد و همچنین به دلیل نقش بافت کلیه در عملکرد فیزیولوژیکی بدن، انجام تحقیقات بیشتر در این زمینه امری ضروری بنظر می‌رسد

کلیدواژه‌ها

موضوعات

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فیزیولوژی ورزشی
دوره 16، شماره 61
فروردین 1403
صفحه 31-46

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سابقه مقاله

  • تاریخ دریافت: 29 آذر 1402
  • تاریخ بازنگری: 27 اردیبهشت 1403
  • تاریخ پذیرش: 13 تیر 1403

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