تأثیر ترکیب تمرین تناوبی با شدت بالا و روزه‌داری متناوب بر مقادیر پروتئینی PGC-1α و UCP-1 بافت چربی احشایی رت های تحت تغذیه با رژیم غذایی پرچرب و پرکربوهیدرات

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان ایران

2 مرکز تحقیقات فیزیولوژی، دانشگاه علوم پزشکی ایران، تهران، ایران.

چکیده
اهداف: مداخلات تغذیه‌ای و فعالیت ورزشی از راهبردهای اصلی مقابله‌ای و درمان چاقی است. پژوهش حاضر با هدف یافتن جنبه جدیدی از تأثیر تمرین تناوبی با شدت بالا (HIIT) و روزه‌داری متناوب (ADF) بر مقادیر پروتئین‌های مرتبط با قهوه‌ای شدن بافت چربی احشایی رت‌های تحت تغذیه با رژیم غذایی پرچرب و پرکربوهیدرات (HFD+HC) انجام شد. مواد و روش‌ها: روش پژوهش حاضر از نوع تجربی، با طرح پس‌آزمون با کنترل بود. بدین منظور 40 سر رت نر اسپراگ‌داولی به‌طور تصادفی به پنج گروه هشت‌تایی تقسیم شدند: 1) رژیم غذایی استاندارد (ND)، 2) HFD+HC، 3) HFD+HC+HIIT، 4) HFD+HC+ADF و 5) HFD+HC+HIIT+ADF. رت‌های HIIT پنج روز در هفته و در هفت تناوب چهار دقیقه‌ای با90ـ85 درصد حداکثر سرعت (Smax) و تناوب‌های استراحتی دو دقیقه‌ای با 50 درصد Smax دویدند. ADF به‌صورت یک روز در میان انجام می‌شد. مقادیر پروتئین PGC-1α و UCP-1 بافت چربی با تکنیک وسترن بلات ارزیابی شد. از t مستقل، ANOVA و آزمون توکی برای تجزیه و تحلیل داده‌ها استفاده شد. یافته‌ها: HFD+HC منجر به افزایش معنی‌دار وزن شد. مقادیر پروتئین‌های PGC-1α و UCP-1 در گروه HFD+HC کاهش معنی‌داری یافت (05/0p<). در گروه‌های HIIT، ADF و HIIT+ADF وزن کاهش معنی‌داری یافت. در گروه-های HIIT و HIIT+ADF مقادیر پروتئینی PGC-1α افزایش معنی‌داری یافت (05/0p<). با این حال، مقادیر پروتئینی UCP-1 تفاوت معنی‌داری بین گروه‌های مداخله نداشت (05/0p>). نتیجه‌گیری: HFD+HC منجر به افزایش وزن می‌شود. در مقابل، HIIT، ADF و به‌ویژه HIIT+ADF علاوه‌بر کاهش وزن منجر به افزایش مقادیر پروتئین PGC-1α می‌شود که احتمالاً با بیوژنز میتوکندریایی و افزایش لیپولیز همراه است.

کلیدواژه‌ها

موضوعات

  1. Ogden CL, Carroll MD, Kit BK, Flegal KM. Prevalence of childhood and adult obesity in the United States, 2011-2012. JAMA. 2014;311(8):806-14.
  2. Barranco P, Delgado J, Gallego L, Bobolea I, Pedrosa M, García de Lorenzo A, et al. Asma, obesidad y dieta. Nutr Hosp. 2012;27(1):138-45.
  3. Langin D. Recruitment of brown fat and conversion of white into brown adipocytes: strategies to fight the metabolic complications of obesity? Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2010;1801(3):372-6.
  4. Frühbeck G, Becerril S, Sáinz N, Garrastachu P, García-Velloso MJ. BAT: a new target for human obesity? Trends Pharmacol Sci. 2009;30(8):387-96.
  5. de Souza Marinho T, Ornellas F, Aguila MB, Mandarim-de-Lacerda CA. Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting. Mol Cell Endocrinol. 2020;513:110872.
  6. Shabalina IG, Petrovic N, de Jong JM, Kalinovich AV, Cannon B, Nedergaard J. UCP1 in brite/beige adipose tissue mitochondria is functionally thermogenic. Cell reports. 2013;5(5):1196-203.
  7. Bartelt A, Heeren J. Adipose tissue browning and metabolic health. Nature Reviews Endocrinology. 2014;10(1):24-36.
  8. Machado SA, Pasquarelli-do-Nascimento G, da Silva DS, Farias GR, de Oliveira Santos I, Baptista LB, et al. Browning of the white adipose tissue regulation: New insights into nutritional and metabolic relevance in health and diseases. Nutr Metab. 2022;19(1):1-27.
  9. Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM. A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell. 1998;92(6):829-39.
  10. Gill J, La Merrill MA. An emerging role for epigenetic regulation of Pgc-1α expression in environmentally stimulated brown adipose thermogenesis. Environmental epigenetics. 2017;3(2):dvx009.
  11. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-8.
  12. Khalafi M, Mohebbi H, Symonds ME, Karimi P, Akbari A, Tabari E, et al. The Impact of Moderate-Intensity Continuous or High-Intensity Interval Training on Adipogenesis and Browning of Subcutaneous Adipose Tissue in Obese Male Rats. Nutrients. 2020;12(4).
  13. Habibi Maleki A, Tofighi A, Ghaderi Pakdel F, Tolouei Azar J, ehsani far m. The effect of three different exercise training on blood lipid profile, fetuin-A, and fibroblast growth factor 21 (FGF-21) in visceral adipose tissue of obese rats. Jundishapur Scientific Medical Journal. 2020;19(1):109-22. (In Persian).
  14. Habibi Maleki A, Tofighi A, Ghaderi PF, Tolouei AJ. The effect of 12 weeks of moderate intensity continuous training (mict) on inflammatory and angiogenesis factors of visceral and subcutaneous adipose tissue in obese rats: a semi-experimental study. 2019. (In Persian).
  15. Habibi Maleki A, Tofighi A, Ghaderi Pakdel F, Tolouei azar J. The Effect of 12 Weeks of High Intensity Interval Training and High Intensity Continuous Training on VEGF, PEDF and PAI-1 Levels of Visceral and Subcutaneous Adipose Tissues in Rats fed with High Fat Diet. Sport Physiology & Management Investigations. 2020;12(1):101-20. (In Persian).
  16. Khalafi M, Mohebbi H, Symonds ME, Karimi P, Akbari A, Tabari E, et al. The impact of moderate-intensity continuous or high-intensity interval training on adipogenesis and browning of subcutaneous adipose tissue in obese male rats. Nutrients. 2020;12(4):925.
  17. Motta VF, Bargut TL, Souza-Mello V, Aguila MB, Mandarim-de-Lacerda CA. Browning is activated in the subcutaneous white adipose tissue of mice metabolically challenged with a high-fructose diet submitted to high-intensity interval training. The Journal of Nutritional Biochemistry. 2019;70:164-73.
  18. Barak Y, Nelson MC, Ong ES, Jones YZ, Ruiz-Lozano P, Chien KR, et al. PPARγ is required for placental, cardiac, and adipose tissue development. Mol Cell. 1999;4(4):585-95.
  19. Thivel D, Masurier J, Baquet G, Timmons BW, Pereira B, Berthoin S, et al. High-intensity interval training in overweight and obese children and adolescents: systematic review and meta-analysis. 2018.
  20. Khalafi M, Symonds ME, Habibi Maleki A, Sakhaei MH, Ehsanifar M, Rosenkranz SK. Combined versus independent effects of exercise training and intermittent fasting on body composition and cardiometabolic health in adults: a systematic review and meta-analysis. Nutrition journal. 2024;23(1):7.
  21. Khalafi M, Habibi Maleki A, Ehsanifar M, Symonds ME, Rosenkranz SK. Longer-term effects of intermittent fasting on body composition and cardiometabolic health in adults with overweight and obesity: A systematic review and meta-analysis. Obesity Reviews.n/a(n/a):e13855.
  22. Jensen MD, Ryan DH, Apovian CM, Ard JD, Comuzzie AG, Donato KA, et al. 2013 AHA/ACC/TOS guideline for the management of overweight and obesity in adults: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines and The Obesity Society. J Am Coll Cardiol. 2014;63(25 Part B):2985-3023.
  23. Dedual MA, Wueest S, Borsigova M, Konrad D. Intermittent fasting improves metabolic flexibility in short-term high-fat diet-fed mice. American Journal of Physiology-Endocrinology and Metabolism. 2019;317(5):E773-E82.
  24. Chung H, Chou W, Sears DD, Patterson RE, Webster NJ, Ellies LG. Time-restricted feeding improves insulin resistance and hepatic steatosis in a mouse model of postmenopausal obesity. Metabolism. 2016;65(12):1743-54.
  25. Hatori M, Vollmers C, Zarrinpar A, DiTacchio L, Bushong EA, Gill S, et al. Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell metabolism. 2012;15(6):848-60.
  26. Khalafi M, Habibi Maleki A, Symonds ME, Rosenkranz SK, Rohani H, Ehsanifar M. The effects of intermittent fasting on body composition and cardiometabolic health in adults with prediabetes or type 2 diabetes: A systematic review and meta‐analysis. Diabetes, Obesity and Metabolism. 2024;26(9):3830-41.
  27. Xiao M, Zhang Y, Xu X. Calorie Restriction Combined with High-Intensity Interval Training Promotes Browning of White Adipose Tissue by Activating the PPARγ/PGC-1α/UCP1 Pathway. 2023.
  28. Cho J, Johnson BD, Watt KD, Niven AS, Yeo D, Kim C-H. Exercise training attenuates pulmonary inflammation and mitochondrial dysfunction in a mouse model of high-fat high-carbohydrate-induced NAFLD. BMC Medicine. 2022;20(1):429.
  29. Habibi Maleki A, Tolouei Azar J, Razi M, Tofighi A. The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways. Reproductive Sciences. 2024;31(8):2246-60.
  30. Habibi Maleki A, Tolouei Azar J, Razi M, Tofighi A. The effect of high-intensity interval training (HIIT) on GLUT-3 expression and lactate levels in testicular tissue of obese rats. Journal of Applied Health Studies in Sport Physiology. 2023. (In Persian).
  31. Azar JT, Maleki AH, Moshari S, Razi M. The effect of different types of exercise training on diet-induced obesity in rats, cross-talk between cell cycle proteins and apoptosis in testis. Gene. 2020;754:144850.
  32. Habibi Maleki A, Tolouei Azar J, Razi M, Tofighi A. The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways. Reproductive Sciences. 2024:1-15.
  33. Tabuchi C, Sul HS. Signaling pathways regulating thermogenesis. Frontiers in endocrinology. 2021;12:595020.
  34. KHALAFI M, RAVASI AA, SHABKHIZ F, MORADI M, ZAREI Y. The effects of high intensity interval exercise (HIIE) and moderate intensity continuous exercise (mice) on serum irisin and subcutaneous UCP-1 in diabetic male rats. 2016. (In Persian).
  35. Krauss S, Zhang C-Y, Lowell BB. The mitochondrial uncoupling-protein homologues. Nature reviews Molecular cell biology. 2005;6(3):248-61.
  36. Mostafavian M, Abdi A, Mehrabani J, Barari A. Effect of eight weeks of aerobic progressive training with capsaicin on changes in PGC-1α and UPC-1 expression in visceral adipose tissue of obese rats with diet. Complementary medicine journal. 2020;10(2):106-17.
  37. Stanford KI, Middelbeek RJ, Goodyear LJ. Exercise effects on white adipose tissue: beiging and metabolic adaptations. Diabetes. 2015;64(7):2361-8.
  38. Brandao CFC, de Carvalho FG, Souza AdO, Junqueira‐Franco MVM, Batitucci G, Couto‐Lima CA, et al. Physical training, UCP1 expression, mitochondrial density, and coupling in adipose tissue from women with obesity. Scandinavian journal of medicine & science in sports. 2019;29(11):1699-706.
  39. Marinho TdS, Ornellas F, Aguila MB, Mandarim-de-Lacerda CA. Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting. Molecular and Cellular Endocrinology. 2020;513:110872.
  40. Cardoso ELdS, Cahuê F, Miranda IEF, Sant’Anna MdL, Andrade CBV, Barbosa RAQ, et al. Combined effects of intermittent fasting with swimming-based high intensity intermittent exercise training in Wistar rats. Tissue and Cell. 2023;82:102099.
  41. Goodpaster BH, Sparks LM. Metabolic Flexibility in Health and Disease. Cell Metab. 2017;25(5):1027-36.
  42. Blondin DP, Labbé SM, Phoenix S, Guérin B, Turcotte É E, Richard D, et al. Contributions of white and brown adipose tissues and skeletal muscles to acute cold-induced metabolic responses in healthy men. J Physiol. 2015;593(3):701-14.
  43. Demine S, Renard P, Arnould T. Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases. Cells. 2019;8(8).
  44. Owen BM, Ding X, Morgan DA, Coate KC, Bookout AL, Rahmouni K, et al. FGF21 acts centrally to induce sympathetic nerve activity, energy expenditure, and weight loss. Cell metabolism. 2014;20(4):670-7.
  45. Scheja L, Heeren J. Metabolic interplay between white, beige, brown adipocytes and the liver. Journal of hepatology. 2016;64(5):1176-86.
  46. Fabbiano S, Suárez-Zamorano N, Rigo D, Veyrat-Durebex C, Dokic AS, Colin DJ, et al. Caloric restriction leads to browning of white adipose tissue through type 2 immune signaling. Cell metabolism. 2016;24(3):434-46.
  47. Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, et al. Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Pparγ. Cell. 2012;150(3):620-32.

 

فیزیولوژی ورزشی
دوره 16، شماره 61
فروردین 1403
صفحه 135-152

  • تاریخ دریافت 25 شهریور 1403
  • تاریخ بازنگری 11 آذر 1403
  • تاریخ پذیرش 20 آذر 1403