نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار فیزیولوژی ورزشی، دانشگاه زنجان
2 کارشناس ارشد فیزیولوژی ورزشی کاربردی، دانشگاه زنجان
چکیده
هدف از پژوهش حاضر، بررسی اثر مصرف مکمل فولات طی 10 هفته تمرین مقاومتی بر گرلین معده، سرم و انسولین سرم موش‌های صحرایی نر ویستار بود. بدین‌منظور، 32 سر موش صحرایی نر ویستار با سن هشت هفته و وزن 2/18±4/204 گرم، پس از یک هفته آشناسازی به‌صورت تصادفی (بر‌حسب وزن بدن) به چهار گروه مساوی (هشت سر در هر گروه) کنترل، مکمل، تمرین مقاومتی و تمرین مقاومتی+ مکمل تقسیم شدند. گروه‌های تمرینی به‌مدت 10 هفته(پنج جلسه در هفته) تمرین مقاومتی را بر روی نردبان(دارای 26 پله) با وزنه‌ای معادل 30 درصد وزن بدن خودآغاز نمودند که وزن آن تا هفتۀ آخر به‌تدریج به 250 درصد وزن بدن آن‌ها رسید. قابل‌ذکر است که مکمل‌دهی به‌صورت 10 میلی‌گرم اسیدفولیک محلول در هر لیتر آب مصرفی انجام شد. 48 ساعت پس از آخرین جلسة تمرینی، موش‌ها بی‌هوش گشتند، تشریح شدند و نمونه‌های معده و سرم آن‌ها جمع‌آوری گردید. علاوه‌براین میزان گرلین، انسولین سرم و گرلین معدۀ آن‌ها به‌وسیلۀ کیت الایزا اندازه‌گیری گشت و با روش‌های آماری آنووا و کروسکال والیس تجزیه‌‌و‌تحلیل گردید (0.05>P). نتایج نشان می‌دهد که گرلین سرم در گروه تمرین مقاومتی در‌مقایسه با گروه‌های کنترل (P=0.002)، مکمل (P=0.001) و مقاومتی+ مکمل (P=0.017) به‌طور معناداری کاهش یافته است. مصرف فولات نیز موجب افزایش معنادار در گروه مقاومتی+ مکمل در‌مقایسه با گروه کنترل (P=0.014) و تمرین مقاومتی (P=0.017) شده است. در غلظت انسولین سرم گروه‌های چهارگانه نیز تفاوت معناداری مشاهده نمی‌شود (P=0.673). بر‌اساس یافته‌ها، مصرف فولات در طول تمرینات مقاومتی، گرلین معده را افزایش داده و یا حداقل از کاهش آن در سرم جلوگیری می‌نماید.
کلیدواژه‌ها
موضوعات
- Tavassoli H, Tofighi A, Hossein Panah F, Hedayati M. The effect of one cycle circuit resistance training on plasma acylated ghrelin, insulin and growth hormone in overweight 17-19- year old males. ZUMS Journal. 2014; 22 (93): 38-4. (In Persian).
- Ghanbari-Niaki A, Abednazari H, Tayebi S M, Hossaini-Kakhak A, Kraemer R R. Treadmill training enhances rat agouti-related protein in plasma and reduces ghrelin levels in plasma and soleus muscle. Metabolism. 2009; 58(12): 1747-52. (In Persian).
- George V A, Morganstein A. Effect of moderate intensity exercise on acute energy intake in normal and overweight females. Appetite. 2003; 40(1): 43-6.
- Casanueva F F, Dieguez C. Ghrelin a new hormone90implicated in the regulation of growth hormone secretion and body energy homeostasis. Growth, Genetics and Hormones. 2004; 20(1): 1-8.
- Broom D, Stensel D, Bishop A, Burn H, Miyashita M. Exercise induced suppression of acylated ghrelin in humans. J Appl Physiol. 2007; 102(6): 2156-71.
- Ariyasu H, Takaya K, Tagami T, Ogawa Y, Hosoda K, Akamizu T, et al. Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in human. J Clin Endocrinol Metab. 2001; 86(10): 4753-8.
- Yada T, Dezaki K, Sone H, Koizumi M, Damdindorj B, Nakata M, et al. Ghrelin regulates insulin release and glycaemia: Physiological role and therapeutic potential. Curr Diabetes Rev. 2008; 4(1): 18-23.
- Rashidlamir A, Ghanbari Niaki A, Rahbary Zadeh F. The effect of 6 weeks wrestling based circuit training on plasma ghrelin and some glucoregulatory hormones of well – trained wrestlers. Sport Biosciences. 2009; 1(1): 75-88.
- Kim H J, Lee S, Kim T W, Kim H H, Jeon T Y, Yoon Y S, et al. Effects of exercise-induced weight loss on acylated and unacylated ghrelin in overweight children. Clin Endocrinol (Oxf). 2008; 68(3): 416-22.
- Saghebjoo M, Ghanbari Niaki A, Rajabi H, Fathi R, Hedayati M. Effects of circuit resistance training on plasma ghrelin levels in young women. Iranian Journal of Endocrinology. 2011; 12(5): 44-52. (In Persian).
- Ravussin E, Tschop M, Morales S, Bouchard C, Heiman M L. Plasma ghrelin concentration and energy balance: Overfeeding and negative energy balance studies in twins. J Clin Endocrinol Metab. 2001; 86(9): 4547-51.
- Broom D, Batterham R, King J, Stensel D. Influence of resistance and aerobic exercise on hunger, circulating levels of acylated ghrelin and peptide YY in healthy males. Am J Physical Regul Integr Comp Physical. 2009; 296(1): 29-35.
- Rezaei M, Sabetkasaei M, Kalantari N, Hedayati M, Abadi A, Omidvar N. Effect of folic acid on serum leptin, grehlin concentration, and feed intake in male Wistar rats. Physiol and Pharma. 2009; 14(4): 426-34. (In Persian).
- Namdari M, Abadi A, Taheri S M, Rezaei M, Kalantari N, Omidvar N. M. Effect of folic acid on appetite in children: Ordinal logistic and fuzzy logistic regressions. Nutrition. 2014; 30(3): 274-8. (In Persian).
- Klok M D, Jakobsdottir S, Drent M L. The role of leptin and ghrelin in the regulation of food intake and body weight in humans: A review. Obes Rev. 2007; 8(1): 21-34.
- Kraemer R R, Castracane V D. Exercise and humoral mediators of peripheral energy balance: Ghrelin and adiponectin. Exp Biol Med. 2007; 232(2): 184-94.
- Van der Lely A J, Tschop M, Heiman M L, Ghigo E. Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin. Endocr Rev. 2004; 25(3): 426-57.
- Guo Z F, Zheng X, Qin Y W, Hu J Q, Chen S P, Zhang Z. Circulating preprandial ghrelin to obestatin ratio is increased in human obesity. J Clin Endocrinol Metab. 2007; 92(5): 1875-80.
- Banaeifar A, Gorzi A, Hedayati M, Nabiollahi Z, Rahmani-Moghadam N, KhantanM. Effect of an 8-week resistance training program on acetylcholinesterase activity in rat muscle. Feyz. 2011; 15(4); 316-21. (In Persian).
- Kraemer R R, Durand R J, Hollander D B, Tryniecki J L, Hebert E P, Castracane V D. Ghrelin and other glucoregulatory hormone responses to eccentric and concentric muscle contractions. Endocrine. 2004; 24(1): 93-8.
- Takano H, Morita T, Iida H, Asada K, Kato M, Uno K, et al. Hemodynamic and hormonal responses to a short – term low – intensity resistance exercise with the reduction of muscle blood flow. Eur J Appl Physiol. 2005; 95(1): 65-73.
- Hoseini R, Ghasemi F, Sayyah A, Ghobadi M. The effects of circuit resistance training on plasma levels of peptide YY and ghrelin in male athletes. Tabari Journal of Preventive Medecine. 2015; 1(1): 1-9. (In Persian).
- Burns S F, Broom D R, Miyashita M, Mundy C, Stensel D J. A single session of treadmill running has no effect on plasma total ghrelin concentrations. J Sports Sci. 2007; 25(6): 635-42.
- Dall R, Kanaley J, Hansen T K, Moller N, Christiansen J S, Hosoda H, et al. Plasma ghrelin levels during exercise in healthy subjects and in growth hormone-deficient patients. Eur J Endocrinol. 2002; 147(1): 65-70.
- Ballard T, Melby C, Camus H, Cianciulli M, Pitts J, Schmidt S, Hickey MS. Effect of resistance exercise, with or without carbohydrate supplementation, on plasma ghrelin concentrations and post exercise hunger and food intake. Metabolism. 2009; 58(8): 191-9.
- Hatamizadeh N, Eftekhar H, Shafaghi B, Mohammad K. Effects of folic acid on preschool children's appetite: Randomized triple-blind clinical trial. Pediatr Int. 2007; 49(5): 558-63. (In Persian).
- Hebert K, House J D, Guenter W. Effect of dietary folic acid supplementation on egg folate content and the performance and folate status of two strains of laying hens. Poultry Sci. 2005; 84(10): 1533-8.
- House J D, Braun K, Balance D M, Oconnor C P, Guenter W. The enrichment of eggs with folic acid through supplementation of the laying hen diet. Poultry Sci. 2002; 81(9): 1332-7.
- Saghebjoo M, Fahimi Y, Rostami M A, Afzalpoor M E, Iiegi S, Hedalbyati M. The acute response of glucose and total and acylated plasma ghrelin variables to high intensity resistance exercise, performed in fasted and satiety states in healthy young men. Olympic Novin. 2014; 1(1): 1-8. (In Persian).
- Rostami M A, Saghebjoo M, Afzalpour M E, Hedayati M. The acute response of total plasma ghrelin to high intensity circuit resistance exercise, performed in fasted and high carbohydrate meal states in healthy young men. Journal of Sport in Biomotor Sciences. 2012; 5(1): 67-76. (In Persian).
- khalili S, Nouri R. The effect of eight weeks resistance training on leptin and insulin resistance in obese female. Sci J Hamadan Univ Med Sci. 2013; 20(1): 59-65. (In Persian).
- Taher Z, Hamednia M, Haghighi H. Investigation of effect of one session moderate and heavy resistance exercise on acute and delayed responses of leptin, insulin, cortisol, testosterone and 24- hour energy expenditure in healthy men. Iranian Journal of Endocrinology and Metabolism. 2011(1); 13: 89-98. (In Persian).
- Aghamohammadi V, Pourghassem Gargari B, Aliasgharzadeh A. Effect of folic acid supplementation on indices of glycemic control, insulin resistance and lipid profile in patients with type 2 diabetes mellitus. Iranian Journal of Endocrinology and Metabolism. 2011; 13(4): 354-60. (In Persian).
- Woods S C, Benoit S C, Clegg D J, Seeley R J. Clinical endocrinology and metabolism. Regulation of energy homeostasis by peripheral signals. Best Pract Res Clin Endocrinol Metab. 2004; 18(4): 497-515.
- Messier V, Malita F M, Rabasa Lhoret R, Brochu M, Karelis A D. Association of cardiorespiratory fitness with insulin sensitivity in overweight and obese postmenopausal women: A montreal Ottawa new emerging team study. Metabolism. 2008; 57(9): 1293-8.
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