اثر تمرین استقامتی بر بیان ژن های هموباکس سی هشت (HOXC8) و سی نه (HOXC9) در بافت چربی موش های صحرایی نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه تربیت‌بدنی، دانشکدۀ علوم انسانی، دانشگاه آیت الله العظمی بروجردی (ره)، بروجرد

2 استاد، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت‌بدنی و علوم ورزشی، دانشگاه تهران، تهران

3 کارشناسی‌ارشد، گروه فیزیولوژی ورزشی، دانشکدۀ تربیت‌بدنی و علوم ورزشی، دانشگاه تهران، تهران

4 دانشیار، گروه بیوشیمی، انستیتو پاستور ایران، تهران

چکیده

پروتئین‌های هموباکس سی هشت(HOXC) و سی نه (HOXC9) پروتئین هایی هستند که در نمو بافت چربی نقش دارند. هدف از این پژوهش، بررسی اثر تمرین استقامتی بر بیان ژن های هموباکس سی هشت و سی نه در بافت چربی سفید زیر پوستی بود. برای این منظور، 16 موش (سن هشت نه هفته با میانگین وزن 220 گرم) صحرایی نژاد ویستار به صورت تصادفی به دو گروه؛ 1)کنترل (هشت سر) و 2) تمرین استقامتی (هشت سر) تقسیم شدند. موش‌های گروه‌ تمرینی، به مدت هشت هفته، هفته‌ای پنج جلسه، تحت تمرین استقامتی تداومی بر روی نوارگردان (با میانگین شدت 25 متر در دقیقه و با میانگین مدت 25 دقیقه) قرار گرفتند. برای اندازه‌گیری بیان نسبی ژن‌های هدف در بافت چربی زیرپوستی از روش ریل تایم (Real Time–PCR)استفاده شد. داده‌ها نشان داد که بیان نسبی ژن پروتئین غیر جفت کننده یک (UCP1) در گروه استقامتی در مقایسه با گروه کنترل به صورت معناداری بیشتر بود (P=0.018). با این حال، بیان نسبی ژن های هموباکس سی هشت و سی نه در گروه استقامتی در مقایسه با گروه کنترل از نظر آماری تفاوت معناداری نداشت (P hoxc9=0.36; Phoxc9=0.52). یافته‌های این مطالعه نشان داد که تمرین استقامتی تغییری در بیان ژن‌های هموباکس سی هشت و سی نه در بافت چربی زیرپوستی ایجاد نمی‌کند؛ که احتمالا بیانگر این است که تمرین استقامتی مورد استفاده در این مطالعه، تغییری در ویژگی بافت چربی سفید از نظر قهوه‌ای شدن یا سفید شدن بافت چربی سفید، ایجاد نمی‌کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Endurance Training on Gene Expression of Homeobox C8 (HOXC8) and Homeobox C9 (HOXC9) in Adipose Tissue of Male Wistar Rats

نویسندگان [English]

  • Saeed Daneshyar 1
  • Mohammad Reza Kordi 2
  • Samane Afshari 3
  • Mehdi Kadivar 4
1 Assistant Professor, Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Boroujerd
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Master of Science, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
4 Associate Professor, Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
چکیده [English]

Homeobox C8 (Hoxc8) and Homeobox C9 (Hoxc9) are homeoproteins involving in white adipose tissue development. The aim of current study was to investigate the effect of endurance training on gene expression of Hoxc8 and Hoxc9 gene in subcutaneous white adipose tissue (WAT). For this, 16 wistar rats (5 weeks old; an average of 210 gram) were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training (by average speed of 25 m/min; by average duration of 25 min) on treadmill for eight weeks (5 session pre week). To measure of gene expression of subcutaneous tissue were used the Real Time (RT) –PCR method. Data showed that gene expression of UCP1 was significantly higher in trained group than control (P=0.018). However, the gene expression of Hoxc8 and Hoxc9 were not significantly different between trained and control groups (Phoxc8=0.36; Phoxc9=0.52). The results of this study showed that the endurance training did not change the gene expression of Hoxc8 and Hoxc9 in subcutaneous WAT, probably suggesting that the endurance training could not change the feature of white adipose tissue with respect to whitening and browning of WAT.

کلیدواژه‌ها [English]

  • Exercise training
  • Browning of white adipose tissue
  • Homeoprotein
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