Sport Physiology

Sport Physiology

The Effect of Endurance Training on Gene Expression of Homeobox C8 (HOXC8) and Homeobox C9 (HOXC9) in Adipose Tissue of Male Wistar Rats

Document Type : Research Paper

Authors
Saeed Daneshyar 1
Mohammad Reza Kordi 2
Samane Afshari 3
Mehdi Kadivar 4
1 Assistant Professor, Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Boroujerd
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Master of Science, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
4 Associate Professor, Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
10.22089/spj.2017.3986.1541
Abstract
Homeobox C8 (Hoxc8) and Homeobox C9 (Hoxc9) are homeoproteins involving in white adipose tissue development. The aim of current study was to investigate the effect of endurance training on gene expression of Hoxc8 and Hoxc9 gene in subcutaneous white adipose tissue (WAT). For this, 16 wistar rats (5 weeks old; an average of 210 gram) were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training (by average speed of 25 m/min; by average duration of 25 min) on treadmill for eight weeks (5 session pre week). To measure of gene expression of subcutaneous tissue were used the Real Time (RT) –PCR method. Data showed that gene expression of UCP1 was significantly higher in trained group than control (P=0.018). However, the gene expression of Hoxc8 and Hoxc9 were not significantly different between trained and control groups (Phoxc8=0.36; Phoxc9=0.52). The results of this study showed that the endurance training did not change the gene expression of Hoxc8 and Hoxc9 in subcutaneous WAT, probably suggesting that the endurance training could not change the feature of white adipose tissue with respect to whitening and browning of WAT.
Keywords
Exercise training
Browning of white adipose tissue
Homeoprotein
Subjects
  1. Fruhbeck G, Becerril S, Sainz N, Garrastachu P, Garcia-Velloso MJ. BAT: a new target for human obesity? Trends Pharmacol Sci. 2009;30(8):387-96.
  2. Wronska A, Kmiec Z. Structural and biochemical characteristics of various white adipose tissue depots. Acta Physiol (Oxf). 2012;205(2):194-208.
  3. Harms M, Seale P. Brown and beige fat: development, function and therapeutic potential. Nature Medicine. 2013;19(10):1252-63.
  4. Walden TB.Regulatory factors that reveal three distinct adipocytes : the brown, the white and the brite. Stockholm: The Wenner-Gren Institute, Stockholm University;2010. p. 89.
  5. Walden TB, Hansen IR, Timmons JA, Cannon B, Nedergaard J. Recruited vs. nonrecruited molecular signatures of brown,“brite,” and white adipose tissues. Am J Physiol-Endoc M. 2012;302(1):E19-E31.
  6. Wu J, Cohen P, Spiegelman BM. Adaptive thermogenesis in adipocytes: is beige the new brown? Gene Dev. 2013;27(3):234-50.
  7. Nedergaard J, Golozoubova V, Matthias A, Asadi A, Jacobsson A, Cannon B. UCP1: the only protein able to mediate adaptive non-shivering thermogenesis and metabolic inefficiency. Biochimica et Biophysica Acta. 2001;1504(1):82-106.
  8. Bonet ML, Oliver P, Palou A. Pharmacological and nutritional agents promoting browning of white adipose tissue. Biochim Biophys Acta. 2013;1831(5):969-85.
  9. Lo KA, Sun L. Turning WAT into BAT: a review on regulators controlling the browning of white adipocytes. Bioscience Reports. 2013;33(5):711-39.
  10. Bartelt A, Heeren J. Adipose tissue browning and metabolic health. Nat Rev Endocrinol. 2014;10(1):24-36.
  11. Cypess AM, Kahn CR. Brown fat as a therapy for obesity and diabetes. Curr Opin Endocrinol Diabetes Obes. 2010;17(2):143-9.
  12. Warner A,Mittag J. Breaking BAT: can browning create a better white? J Endocrinol. 2016;228(1):R19-29.
  13. Gehring W, Hiromi Y. Homeotic genes and the homeobox. Annu Rev Genet. 1986;20(1):147-73.
  14. Krumlauf R. Hox genes in vertebrate development. Cell.1994;78(2):191-201.
  15. Gehring WJ, Affolter M, Burglin T. Homeodomain proteins. Annu Rev Biochem. 1994;63(1):487-526.
  16. Nakagami H. The mechanism of white and brown adipocyte differentiation. Diabetes Metab J. 2013;37(2):85-90.
  17. Procino A, Cillo C. The HOX genes network in metabolic diseases. Cell Biology International. 2013;37(11):1145-8.
  18. Cantile M, Procino A, D'Armiento M, Cindolo L, Cillo C. HOX gene network is involved in the transcriptional regulation of in vivo human adipogenesis. Journal of Cellular Physiology. 2003;194(2):225-36.
  19. Mori M, Nakagami H, Rodriguez-Araujo G, Nimura K, Kaneda Y. Essential role for miR-196a in brown adipogenesis of white fat progenitor cells. PLoS Biol. 2012;10(4):e1001314.
  20. Petrovic N, Walden TB, Shabalina IG, Timmons JA, Cannon B, and Nedergaard J. Chronic peroxisome proliferator-activated receptor gamma (PPARgamma) activation of epididymally derived white adipocyte cultures reveals a population of thermogenically competent, UCP1-containing adipocytes molecularly distinct from classic brown adipocytes. J Biol Chem. 2010;285(10):7153-64.
  21. Waldén TB, Hansen IR, Timmons JA, Cannon B, and Nedergaard J. Recruited vs. nonrecruited molecular signatures of brown, “brite,” and white adipose tissues. Am. J. Physiol. Endocrinol. Metab. 2012; 302:E19-E31.
  22. Jeremic N, Chaturvedi P, Tyagi SC. Browning of white fat: novel insight into factors, mechanisms, and therapeutics. J Cell Physiol. 2017;232(1):61-8.
  23. De Matteis R, Lucertini F, Guescini M, Polidori E, Zeppa S, Stocchi V, et al. Exercise as a new physiological stimulus for brown adipose tissue activity. Nutr Metab Cardiovasc Dis. 2013;23(6):582-90.
  24. Xu X, Ying Z, Cai M, Xu Z, Li Y, Jiang SY, et al. Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue. Am J Physiol Regul Integr Comp Physiol. 2011;300(5):R1115-25.
  25. Stanford KI, Middelbeek RJW, Goodyear LJ. Exercise Effects on white adipose tissue: beiging and metabolic adaptations. Diabetes. 2015;64(7):2361-2368.
  26. Ringholm S, Grunnet Knudsen J, Leick L, Lundgaard A, Munk Nielsen M, Pilegaard H. PGC-1alpha is required for exercise-and exercise training-induced UCP1 up-regulation in mouse white adipose tissue. PLoS One. 2013;8(5):e64123.
  27. Knudsen JG, Murholm M, Carey AL, Bienso RS, Basse AL, Allen TL, et al. Role of IL-6 in exercise training- and cold-induced UCP1 expression in subcutaneous white adipose tissue. PLoS One. 2014;9(1):e84910.
  28. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-8.
  29. Reisi J, Rajabi H, Ghaedi K, Marandi S-M, Asady Samani Z, Kazemi Nasab f. Effect of 8 weeks resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats. Sport Physiology. 2016;7(28):117-30. (In Persian).
  30. Afshari S, Mohammad-Amoli M, Daneshyar S. Comparison of moderate and high volume aerobic training on gene expression of uncoupling protein 1  (UCP-1) in  subcutaneous white adipose tissue of wistar rats. Iranian Journal of Endocrinology and Metabolism. 2017;19(1):34-40. (In Persian).
  31. Nikooie R, Rajabi H, Gharakhanlu R, Atabi F, Omidfar K, Aveseh M, et al. Exercise-induced changes of MCT1 in cardiac and skeletal muscles of diabetic rats induced by high-fat diet and STZ. J Physiol Biochem. 2013;69(4):865-77.
  32. Galbes O, Goret L, Caillaud C, Mercier J, Obert P, Candau R, et al. Combined effects of hypoxia and endurance training on lipid metabolism in rat skeletal muscle. Acta Physiologica. 2008;193(2):163-173.
  33. Ropper AE, Thakor DK, Han I, Yu D, Zeng X, Anderson JE, et al. Defining recovery neurobiology of injured spinal cord by synthetic matrix-assisted hMSC implantation. Proceedings of the National Academy of Sciences. 2017;114(5):E820-E829.
  34. Daneshyar S, Kordi MR, Gaeini AA, Kadivar M, Afshari S. The Effect of endurance training on gene expression of uncoupling protein 1(UCP-1) in retroperitoneal white adipose tissue of male wistar rats. Razi Journal of Medical Sciences. 2015;22(136):35-45. (In Persian).
  35. Trevellin E, Scorzeto M, Olivieri M, Granzotto M, Valerio A, Tedesco L, et al. Exercise training induces mitochondrial biogenesis and glucose uptake in subcutaneous adipose tissue through eNOS-dependent mechanisms. Diabetes. 2014;63(8):2800-2811.
  36. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-8.
  37. Camera DM, Anderson MJ, Hawley JA, Carey AL. Short-term endurance training does not alter the oxidative capacity of human subcutaneous adipose tissue. Eur J Appl Physiol. 2010;109(2):307-16.
  38. Pino MF, Parsons SA, Smith SR, Sparks LM. Active individuals have high mitochondrial content and oxidative markers in their abdominal subcutaneous adipose tissue. Obesity (Silver Spring). 2016;24(12):2467-70.
  39. Vosselman MJ, Hoeks J, Brans B, Pallubinsky H, Nascimento EB, van der Lans AA, et al. Low brown adipose tissue activity in endurance-trained compared with lean sedentary men. Int J Obes (Lond). 2015;39(12):1696-702.
  40. Yamamoto Y, Gesta S, Lee KY, Tran TT, Saadatirad P, and Kahn CR. Adipose depots possess unique developmental gene signatures. Obesity (Silver Spring). 2010;18(5):872-8.

 

Sport Physiology
Volume 13, Issue 49
Winter 2021
Pages 17-38

  • Receive Date 17 April 2017
  • Revise Date 10 October 2017
  • Accept Date 17 October 2017