مقایسه تأثیر تمرین استقامتی کم شدت (LIET) و تمرین تناوبی شدید (HIIT) بر بیان ژن Hig2 و محتوای تری-گلیسیرید چربی احشایی رت‌های مبتلا به کبد چرب غیرالکلی (NAFLD)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه شهرکرد، ایران

2 دانشیار گروه فیزیولوژی ورزشی، دانشگاه شهرکرد، ایران

3 دانشیار گروه بیوشیمی، دانشکدۀ داروسازی و علوم دارویی دانشگاه علوم پزشکی اصفهان، ایران

چکیده

هدف از این پژوهش مقایسه تأثیر هشت هفته تمرینات LIET و HIIT بر میزان بیان ژن HIG2 و محتوایTG بافت چربی احشایی موش‌های صحرایی نر مبتلا به NAFLD بود. این پژوهش بر روی 40 سر موش نر نژاد ویستار مبتلا شده بهNAFLD انجام شد. موش‌ها به گروه‌های ده‌تایی کنترل سالم و گروه های کنترل LIET و HIIT (رژیم غذایی پر چرب) تقسیم شدند. مصرف رژیم غذایی پرچرب پس از گذشت 16 هفته تا پایان دوره تمرین ادامه داشت. پروتکل تمرینHIIT باشدت 90 % و پروتکل تمرینی LIET شامل دویدن باشدت 45% حداکثر سرعت بیشینه بود. سنجش بیان‌ژن HIG2 با استفاده از تکنیک Real-time PCR و سنجش TG بر روی دستگاه اتو آنالیزور (مدل BT3000) انجام شد. تحلیل داده‌ها نشان داد که تفاوت معنی‌داری در میزان بیان ژن HIG2 بین گروه‌های تمرینی در مقایسه با گروه کنترل وجود ندارد (05/0P>). افزایش معنی‌داری در میزان بیان ژن HIG2 صرفاً در گروه کنترل (039/P=0) نسبت به گروه شم مشاهده شد. تفاوت معنی‌داری در میزان محتوایTG بافت چربی احشایی بین گروه کنترل و تمرین استقامتی کم شدت (001/P=0) و بین گروه کنترل و تمرین تناوبی شدید (001/P=0) و بین تمرین استقامتی کم شدت و تمرین تناوبی شدید (003/P=0) مشاهده شد.مشاهده شد. علاوه براین، می توان چنین نتیجه گرفت که ادامه رژیم پرچرب در طول تمرین و نیز تاثیر تمرینات بر میزان TG بافت چربی احشایی و عدم تاثیر بر بیان ژن HIG2، به نظر می‌رسد که هر دو نوع تمرین مستقل از مسیر HIG2 بر محتوای چربی احشایی در رت‌های مبتلا بهNAFLD تاثیر داشتند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Low Intensity Endurance Training and High Intensity Interval Training on HIG2 Gene Expression and Visceral Adipose Tissue Triglyceride Content in Rats with NAFLD

نویسندگان [English]

  • ahmad heidari shareza 1
  • Akbar Azamian Jazi 2
  • ebrahim banitalebi 2
  • abasali palizban 3
1 Ph.D. Student of Exercise Physiology, Department of Sport Sciences, Shahrekord University, Shahrekord, Iran
2 Associate Professor of Exercise Physiology, Department of Sport Sciences, Shahrekord University, Shahrekord, Iran
3 Associate Professor in Clinical Biochemistry, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

The main purpose of this study was to compare the effect of an eight-week LIET and HIIT, on the amount of HIG2 gene and TG content of visceral lipid tissue in wistar male rats affected by NAFLD. The present research was performed on 40 wistar rats affected by NAFLD. The rats were divided into four-ten groups Healthy Control (Standard Diet), control, LIET and HIIT (High fat diet). After 16-weeks of using special diet and end of practical or training period. HIIT training protocol consisted 90% and The LIET training protocol composed of running with 45% maximum speed intensity. HIG2 gene expression by real-time PCR technique and TG measurements visceral lipid tissue was done by auto analyzer (BT3000 model). Analysis of data revealed that there is no significant difference in HIG2 gene expression among training groups compared to control groups (p=0.05). The increasing significance was observed in HIG2 gene expression amount merely in control group (p=0/039) compared to experimental group. There was a significant deference in the amount of TG content between control group and LIET group (p=0/001) and between control group and HIIT group (p=0/001) and between LIET and HIIT group (p= 0/003). It can be concluded that considerable continuation of fatty diet during the training and the influence of exercises on the amount of TG; and on effect on HIG2 gene expression demonstrate that each of the tow training program, independent of HIG2 gene, had an effect on visceral fat content in NAFLD affected rats.

کلیدواژه‌ها [English]

  • Intra-Abdominal Fat
  • High-intensity interval training
  • hypoxia-inducible protein 2
  • Triglyceride
  • Non-alcoholic fatty liver disease
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