اثر هشت هفته تمرین هوازی توأم با مصرف اتانول بر بیان ژن Nrf2 در عضلۀ قلب و برخی از شاخص‌های آنتی‌اکسیدانی موش صحرایی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 استاد گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

3 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

4 دانش‌آموخته دکتری فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

چکیده

در ارتباط با تأثیر ترکیب تمرینات ورزشی و مصرف الکل بر تنظیم کننده‌های سیستم دفاع آنتی اکسیدانی به ویژه فاکتور هسته‌ای اریتروئید 2 مرتبط با فاکتور2 (Nrf2) در عضله قلب مطالعات محدودی انجام شده است. هدف مطالعه حاضر، بررسی اثر هشت هفته تمرین هوازی توأم با مصرف اتانول بر بیان ژنNrf2 عضله قلب و شاخص‌های آنتی‌اکسیدانی پلاسما در موش صحرایی نر بود. تعداد 32 سر موش صحرایی با میانگین وزنی6±230 گرم به چهار گروه کنترل، تمرین هوازی، اتانول20% با دوز 4 گرم بر کیلوگرم وزن بدن و اتانول به همراه تمرین تقسیم شدند. در پایان دوره سطوح میزان بیان ژن Nrf2 و میزان ظرفیت تام آنتی‌اکسیدانی (TCA) و مالون دی‌آلدئید (MDA) مورد ارزیابی قرار گرفتند. داده‌ها توسط آزمون تحلیل واریانس دو سویه و در سطح معنی‌داری 0.05≥p تجزیه و تحلیل شدند. نتایج نشان داد که تمرین هوازی اثر معنی‌داری در بیان ژن Nrf2 دارد (P=0.0068). نتایج عدم تأثیر معنی‌دار مصرف اتانول(P=0.312) و اثر تعاملی بین تمرین هوازی و مصرف اتانول (P=0.237) را در بیان ژن Nrf2 نشان دادند. تمرین هوازی بطور معنی‌داری بیان ژن Nrf2 را در گروه‌های تمرین و تمرین به همراه اتانول نسبت به گروه کنترل افزایش داد. مصرف اتانول بطور معنی‌داری سبب کاهش میزان TCA و افزایش MDA در مقایسه با سایر گروه‌ها شد. یافته‌ها نشان داد که مصرف اتانول به ترتیب سبب کاهش و افزایش سطوح پلاسمایی TAC و MDA می‌گردد. در مقابل انجام تمرین هوازی از طریق افزایش سطوح TAC و افزایش بیان ژن Nrf2 منجر به کاهش آسیب اکسیداتیو ناشی از مصرف اتانول شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Effect of Aerobic Exercise and Ethanol Consumption on Nrf2 Gene Expression in Heart Tissue and Some Antioxidant Indices in Male Rat

نویسندگان [English]

  • Zahra Frajtabar 1
  • Rozita Fathi 2
  • Khadijeh Nasiri 3
  • Farhad Ahmadi 4
1 M.Sc. Graduate in Exercise Physiology, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
2 Professor, Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran
3 Assistance Professor, Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran
4 Ph.D. Graduate in Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran
چکیده [English]

There have been limited studies on the effect of the combination of aerobic exercise and alcohol consumption on regulators of antioxidant defense systems, especially erythroid nuclear factor 2 associated with factor 2 (Nrf2) in heart muscle.The aim of this study was to investigate the effect of eight weeks of aerobic exercise with ethanol consumption on Nrf2 gene expression in heart tissue and antioxidant parameters of plasma in male rats. A number of 32 rats with a weighing average of 230 ± 6 g were divided into four groups control, aerobic exercise, ethanol 20% at a dose of 4 g/kgbw, and ethanol+ aerobic exercise. At the end of the period, levels of Nrf2 gene expression and total capacity antioxidant and malondialdehyde were evaluated. Data were analyzed by two-way ANOVA at the significant level of p≤0.05.The results showed that aerobic exercise had a significant effect on Nrf2 gene expression (P=0.0068).The results showed no significant effect of ethanol consumption (P=0.312) and interaction effect between aerobic exercise and ethanol consumption (P=0.237) on Nrf2 gene expression. Aerobic exercise significantly increased the expression of Nrf2 gene in the aerobic exercise and ethanol+ aerobic exercise groups compared to the control. Ethanol consumption significantly reduced TCA and increased MDA levels compared to other groups.The findings showed that ethanol consumption decreased and increased plasma levels of TAC and MDA, respectively. In contrast, aerobic exercise through increasing TAC levels and increase Nrf2 gene expression leads to a decrease in the oxidative damage caused by ethanol consumption.

کلیدواژه‌ها [English]

  • Ethanol
  • Aerobic Exercise
  • Erythroid Nuclear Factor 2 Related to Factor (2) (Nrf2)
  • gene expression
  • antioxidant
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