تأثیر یک دوره تمرین ترکیبی و بازی در محیط غنی‌شده در دورۀ پیش از بلوغ بر ساختار هیپوکمپ موش‌های صحرایی بزرگسال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه شهید بهشتی

2 استاد مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی شهید بهشتی

3 استادیار فیزیولوژی ورزشی، دانشگاه شهید بهشتی

چکیده

حفظ ساختار و عملکرد مغز در طول زندگی از اهمیت بالایی برخوردار است. فرآیندهای کلیدی توسعه‌ مغزی در دوره-های بحرانی رشد به ویژه در دوره پیش از بلوغ برجسته هستند. از این رو مداخلات محیطی در طی این دوره حساس رشدی، ممکن است به تغییرات قابل توجه و ماندگار در ساختارهای در حال رشد مانند هیپوکمپ منجر شوند. در این مطالعه، تاثیر تمرین ترکیبی و بازی در محیط غنی شده پیش از بلوغ بر ساختار هیپوکمپ موش های صحرایی بزرگسال مورد ارزیابی قرار گرفت. بدین منظور، تحقیق به صورت طولی انجام شد و 27 سر موش‌ صحرایی نر پس از دوره شیردهی به سه گروه تمرین ترکیبی، بازی در محیط غنی‌شده و کنترل تقسیم شدند. مداخلات به مدت سه هفته در دوران قبل از بلوغ (48-28 روزگی) اجرا شدند و در ادامه تا دوره بزرگسالی (85-48 روزگی) همه حیوانات بدون مداخله درون قفس-های استاندارد نگهداری شدند. پس از این دوران، مراحل تشریح و بافت‌برداری هیپوکمپ انجام شدند. روش وسترن بلات برای اندازه‌گیری عامل رشد عصبی مشتق از مغز (BDNF) و رنگ‌آمیزی نیسل برای ارزیابی تعداد سلول‌ها در نواحی هیپوکمپ استفاده شدند. نتایج آزمون آنوا یک‌طرفه نشان دادند که سطوح پروتئین BDNF (P

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of combined training and play in an enriched environment during pre-pubertal period on hippocampal structure of adult rats

نویسندگان [English]

  • Samira Rostami 1
  • Abbas Haghparast 2
  • Rana Fayazmilani 3
1 Ph.D. Student in Exercise Physiology, Shahid Beheshti University
2 Professor of Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences
3 Assistant Professor of Exercise Physiology, Shahid Beheshti University
چکیده [English]

It is important to maintain the structure and function of the brain throughout life. The key brain development processes occur in the critical periods, especially in the pre-pubertal period. Thus, environmental interventions during this critical period may lead to significant and lasting changes in the developing structures such as the hippocampus. Here, we studied the effect of combined training and play in an enriched environment during pre-pubertal period on the hippocampal structure of adult rats. For this purpose, a longitudinal study was performed. After weaning, 27 male rats were divided into three groups of combined training, play in an enriched environment, and control. Interventions were performed for three weeks (28-48 postnatal days). Subsequently, all animals were kept without intervention in the standard cages until adulthood (48-85 postnatal days). After this period, the animals were sacrificed and hippocampal tissues were removed. Western blotting and Nissl staining methods were used to assess Brain-derived neurotrophic factor (BDNF) and the number of cells in the hippocampus, respectively. The results showed that BDNF levels (P

کلیدواژه‌ها [English]

  • Brain Development
  • Combined Training
  • Play in an Enriched Environment
  • Pre-pubertal Period
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