تأثیر تمرین تناوبی با شدت بالا (HIIT) و مکمل‌دهی Q10 بر محتوی پروتئین PGC-1α و فعالیت آنزیم سیترات سنتاز در عضله نعلی موش‌های صحرائی نر چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه علوم ورزشی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

4 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکدۀ علوم اجتماعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران

چکیده

چاقی بیوژنز میتوکندری عضلات را تضعیف می‌کند و در مورد تاثیر HIIT و Q10 بر آن نیاز به بررسی وجود دارد. هدف پژوهش بررسی اثر توام تمرین HIIT و مکمل Q10 بر دو شاخص بیوژنز میتوکندری در عضله نعلی موش‌های نر چاق بود. 48 موش صحرائی نر 16 هفته-ای]وزن گروه‌های چاق 272 تا372 گرم(36/22±8/308گرم)؛ وزن گروه وزن معمولی 140تا197 گرم (21/226/158گرم)[،به شش گروه شامل وزن معمولی، چاق مرجع، چاق کنترل، چاق HIIT، چاق Q10 و چاق توام تقسیم شدند. چاقی با رژیم پرچرب القا شد. مصرف Q10 روزانه به مقدار mg/kg.bw 500 و تمرین HIIT (10 وهله‌ فعالیت4 دقیقه‌ای با شدت 90-85 درصد v VO2 peak با 2 دقیقه‌ استراحت) به مدت12 هفته انجام شدند. متغیرها به روش وسترن بلات و اسپکتروفتومتری اندازه‌گیری شدند و داده‌ها با تحلیل واریانس یک‌راهه مقایسه شدند. در گروه‌‌های چاق مرجع و کنترل، مقدار پروتئین PGC-1α عضله نعلی کمتر از گروه وزن معمولی(به ترتیب P=0.001 و P=0.003) و در عوض فعالیت آنزیم سیترات سنتاز بیشتر بود (به ترتیبP=0.039وP=0.031).HIIT (0.001=P)، Q10 (0.001=P) و اثر توام(0.001=P) مقدار PGC-1α را از گروه وزن معمولی(نیز فراتر بردند که از این لحاظ اثر HIIT بیشتر از Q10 بود(0.017=P). اما هیچ یک از مداخلات، فعالیت CS را تغییر ندادند(P>0.05). می‌توان گفت چاقی بیوژنز میتوکندری عضله را تضعیف می‌کند و HIIT به‌طور مناسبی سبب جبران این وضعیت و حتی ارتقای آن نسبت به موش‌های وزن معمولی می‌شود. مکمل Q10 نیز دارای اثرات مفید اما ضعیف‌تر از HIIT است. با این‌حال، به دلیل کمبود شواهد و محدودیت‌ها هنوز نیاز به بررسی‌ باقی است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of HIIT and Q10 supplementation on soleus muscle PGC-1α level citrate synthase activity in obese male rats

نویسندگان [English]

  • Masoud Rafati Bonab 1
  • jabbar bashiri 2
  • roghayeh poozesh jadidi 3
  • hassan pourrazi 4
1 Ph.D. Student of Exercise Physiology, Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Associate Professor of Exercise Physiology, Department of Sport Sciences, Tabriz branch, Islamic Azad University, Tabriz, Iran
3 Assistance Professor of Exercise Physiology, Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 Assistance Professor of Exercise Physiology, Department of Sport Sciences, Imam Khomeini International University, Qazvin, Iran
چکیده [English]

Obesity suppreses muscle mitochondorial biogenesis and the effects of HIIT and Q10 in this area remains to be more investigated. The aim was to investigate the concomitant effects of HIIT and Q10 supplementation on two indices of mitochondorial biogenesis in obese male rats’ skeletal muscle.48 male rats[16 weeks, obese group weight: 272to372gr(308.8±22.36gr); normal weight: 140 to 197gr (158.622.21gr)] were randomized into six groups including normal weight, obese reference, obese control, obese HIIT, obese Q10 and obese HIIT concomitany with Q10 groups. Obesity was induced by high fat diet. Q10 supplentation 500 mg/kg.bw.day and HIIT(10 activity bouts for 4 min at 85-90% of v VO2 peak with 2min rest intervals) were done for 12 weeks. Western blot and spectrophotometery methods were respectively used to quantify the variables and the data were analyzed using one-way ANOVA. A lower soleus PGC-1α content were observed in obese reference(P=0.001) and also obese control(P=0.003) groups compared to normal weight group with higher citrate synthase activity (P=0.039,P=0.031 respectively) in both of them. HIIT(P=0.001), Q10(P=0.001) and their concomitment (P=0.001) elevated PGC-1α expression to levels even higher than normal weight group, with more efficacy were noted for HIIT rather than Q10(P=0.017). None of interventions changed CS activity (P>0.05). It can be concluded that obesity suppreses muscle mitochondorial biogenesis and HIIT efficiently compensates this condition and even elevates muscle mitochondorial biogenesis compared to normal weight rats. Q10 supplementation has also beneficial effects, although weaker than HIIT. However, more investigations remain to be done because of similar evidence and study limmitations.

کلیدواژه‌ها [English]

  • HIIT
  • Q10
  • obesity
  • soleus muscle
  • Mitochondorial Biogenesis
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