نوع مقاله : مقاله پژوهشی
نویسندگان
1 دکتری فیزیولوژی ورزشی، دانشکدة علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
2 استاد فیزیولوژی ورزشی، دانشکدة علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
3 دانشیار بیوشیمی، مرکز تحقیقات سلولی-مولکولی، دانشکدة پزشکی، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران و دانشیار بیوشیمی، گروه میکروبیولوژی و ژنتیک مولکولی، دانشگاه ایالتی میشیگان، آمریکا
4 استاد بیوشیمی، مرکز تحقیقات سلولی مولکولی غدد درون‌ریز، پژوهشکدة علوم غدد درون‌ریز و متابولیسم، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
5 دانشجوی دکتری فیزیولوژی ورزشی، دانشکدة علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
چکیده
مطالعات نشان می دهند که تمرین ورزشی و ترکیبات فعال زیستی زعفران می توانند باعث کاهش آپوپتوز و کاشکسی ناشی از سرطان شوند. پژوهش حاضر با هدف بررسی اثر چهار هفته تمرین تناوبی شدید (HIIT) و مصرف عصارة آبی زعفران (SAE) بر بیان برخی ژن های مرتبط با کاشکسی (SIRT1، hTERT و p53) در عضلة دوقلوی موش های حامل ردة سلولی 4T1 سرطان پستان انجام شد؛ براین‌اساس، 44 سر موش BALB/c ماده به طور تصادفی در گروه های تمرین تناوبی شدید (HIIT)، عصارة آبی زعفران (SAE)، تمرین تناوبی شدید + عصارة آبی زعفران (HIIT+SAE)، کنترل و شم قرار گرفتند. پس از 48 ساعت از آخرین جلسة مداخله، عضلة دوقلو با روش جراحی برای آنالیزهای بعدی برداشته شد. نتایج نشان داد بیان ژن SIRT1 در گروه HIIT بهطور معناداری از گروه کنترل و HIIT+SAE بالاتر بود (به‌ترتیب 0.03P = و 0.02P = ). همچنین بیان ژن hTERT در گروه HIIT به طور معناداری از سایر گروههای مطالعه‌شده بالاتر بود (P < 0.05) و درگروه SAE بهطور معناداری از گروه HIIT+SAE بالاتر بود (P = 0.01). علاوه‌براین، بیان ژن p53 در گروههای HIIT و HIIT+SAE به طور معناداری از گروه SAE پایین‌تر بود (به‌ترتیب 0.003 P = و 0.004P =). براساس نتایج، به‌نظر میرسد HIIT احتمالاً نقش مثبتی در کاهش اثرات کاشکسی ناشی از سرطان با تنظیم بیان بالاتر ژنهای SIRT1 و hTERT و بیان پایین تر ژن p53 دارد و می تواند در کاهش تحلیل عضلات ناشی از سرطان مؤثر باشد.
کلیدواژه‌ها
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