نانو ذرات نقره و تغییرات ساختاری بافت مغز رت‌های نر درپی پیش‌آماده‌سازی هوازی و بی‌هوازی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی‌ارشد فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد شاهرود، دانشگاه آزاد اسلامی، شاهرود، ایران

2 استادیار فیزیولوژی ورزشی، واحد شاهرود، دانشگاه آزاد اسلامی، شاهرود، ایران

چکیده

استفاده از نانو ذرات رو به افزایش است و می‌تواند موجب مسمومیت‌های عصبی و تغییرات بافت مغز شود. از طرفی پیش‌آماده‌سازی بدنی مقاومت سلول‌های عصبی را افزایش می‌دهد؛ بنابراین این پژوهش با هدف بررسی میزان تغییرات ساختاری بافت مغز در اثر تزریق نانو نقره درپی دو پروتکل تمرین انجام شد. تعداد 30 سر رت نر نژاد ویستار به شش گروه کنترل سالم، نانو نقره، پروتکل هوازی، پروتکل بی‌هوازی، نانو نقره + پروتکل هوازی، نانو نقره + پروتکل بی‌هوازی به‌صورت تصادفی ساده تقسیم شدند. تزریق درون‌صفاقی نانو نقره پس از اجرای 10 هفته پروتکل هوازی و بی‌هوازی به ازای 10 درصد وزن بدن هر رت در پنج نوبت بود و پس از 48 ساعت از آخرین تزریق رتها بی‌هوش شدند و نمونه‌گیریانجام شد. سپس نمونه‌ها با رنگ‌آمیزی هماتوکسیلین-ائوزین و توسط میکروسکوپ نوری عکس‌برداری و مطالعه شدند. نتایج نشان داد پیش‌آماده‌سازی به‌صورت معناداری بر وزن (0.045= p ) و میزان مسافت طی‌شده در آزمون استقامتی پیش‌رونده (0.000 = p ) اثرگذار بود. همچنین نانو نقره موجب التهاب (0.000 = p ) و دژنراسیون نورونی (0.000= p ) در رتهای دریافت‌کنندة نانو نقره شد، اما در گروه های تمرینی، التهاب (0.001 = p ) و دژنراسیون (0.001= p ) تقلیل یافته بود؛ اگرچه تفاوت معناداری میان دو نوع پروتکل در التهاب (0.928= p ) و دژنراسیوننورونی (0.875 = p ) وجود نداشت. به‌نظر می‌رسد پیش‌آماده‌سازی تمرینی میتواند در کاهش درجة تخریب بافت مغزی ناشی از مصرف نانو نقره مؤثر باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Silver Nanoparticles and Structural Changes in Brain Tissue of Male rats Following Aerobic and Anaerobic Preconditioning

نویسندگان [English]

  • Afrooz Motahedi 1
  • Seyed Javad Ziaolhagh 2
1 M.Sc. of Exercise Physiology, Faculty of Basic Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2 Assistant Professor of Exercise Physiology, Shahrood Branch, Islamic Azad University, Shahrood, Iran
چکیده [English]

The use of nanoparticles is increasing and can cause neurotoxicity and brain tissue changes. On the other hand, pre-conditioning improves neuronal protections. Therefore, the aim of this study was to investigate the structural changes in brain tissue following Nano silver injections following the two protocols. 30 male Wistar rats were divided in 6 groups of healthy control, nano silver, aerobic protocol, anaerobic protocol, nano silver + aerobic protocol, nano silver + anaerobic protocol. Intraperitoneal injection of nano silver after 10 weeks of aerobic and anaerobic treatments was performed 10% of each rat's body weight in 5 turns. After 48 hours of the last infusion, the rats were indifferent and sampled. The samples were then photographed and stained with Hematoxylin-Eosin and were studied by optical microscopy. The results of this study indicated that pre-conditioning had a significant effect on weight (P = 0.045) and distance traveled in progressive endurance test (P = 0.000). Nanosilver also caused inflammation (P = 0.000) and neuron degeneration (P = 0.000) of brain tissue in nanosilver rats. However, in the training groups, inflammation (P = 0.001) and degeneration (P = 0.001) were reduced, although there was no significant difference between the protocols in degeneration (P = 0.875) and inflammation (P = 0.928). It seems that pre-conditioning of exercise can be effective in reducing the degree of degradation of brain tissue due to the use of silver nanoparticles.

کلیدواژه‌ها [English]

  • silver nanoparticles
  • aerobic training pre-conditioning
  • anaerobic training pre-conditioning
  • neuron degeneration
  • neuron inflammation
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