تأثیر چهار هفته تمرین هوازی بر بیان ژن فاکتور تغذیه‌ای مشتق‌شده از سلول‌های گلیال، TNF-α و عوامل شناختی در هیپوکمپ رت‌های مبتلا به بیماری آلزایمر القاشده با آمیلویید بتا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکدة علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

2 استاد فیزیولوژی ورزشی، دانشکدة علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

3 دانشیارفیزیولوژی ورزشی، دانشکدة علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

4 دکتری فیزیولوژی ورزشی، دانشکدة علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

زمینه و هدف : بیماری آلزایمر شایعترین شکل زوال عقل است و آمیلوئیدبتا نقشی در بیماری زایی آن ایفا میکنند. در سالهای اخیر ورزش منظم به عنوان یکی از سازوکارهای غیردارویی مهم برای مقابل با بیماری آلزایمر مطرح شده است. هدف از مطالعه بررسی تاثیر 4 هفته تمرین هوازی بر روی بیان ژن GDNF در رت های مبتلا به آلزایمر القا شده با Aβ1-42 بود.
روش کار: آزمودنی های پژوهش شامل 56 سر رت نر ویستار 8 هفته ای با میانگین وزن 20±190 گرم بودند که به طور تصادفی به چهارگروه تقسیم شدند: گروه ورزش، گروه آلزایمر+ ورزش، گروه آلزایمر و گروه کنترل. Aβ1-42 به درون هیپوکمپ تزریق شد و 7 روز بعد از جراحی و توسعه بیماری آلزایمر رت های هر گروه به طور تصادفی قربانی شدند و گروهی تحت آزمون رفتاری قرار گرفتند. برای اندازه گیری بیان ژن GDNFبا استفاده از روش کمی Real time-PCR استفاده شد.
نتایج: براساس مقایسه سطوح بیان ژن در چهار گروه مورد مطالعه، بیان ژن GDNF در بین گروههای تحقیق اختلاف معنی داری دارند بطوریکه ژن GDNF در گروه تمرین بیشترین و در گروه آمیلوئید بتا کمترین سطح بیان را داشته است (p <0.001). یادگیری و حافظه فضایی در گروه ورزش به طور معنی داری بهتر از گروه آلزایمری بود (p <0.001).
نتیجه گیری: به نظر می رسد تمرین هوازی می تواند نقش بسزایی در بهبود یادگیری و همچنین افزایش بیان ژن های عامل تغذیه ای عصب در هیپوکمپ آلزایمری خصوصا GDNF داشته باشد که نهایتا به بهتر شدن حافظه و یادگیری کمک می کند.

کلیدواژه‌ها


عنوان مقاله [English]

the effect of 4 weeks exercise on mRNA of GDNF in rat’s hippocampus with AD induced by Aβ1-42 injection

نویسندگان [English]

  • peyman ghasemi 1
  • reza gharakhanlo 2
  • mahdieh molanouri shamsi 3
  • davar khodadadi 4
1 Ph.D. student of Sport physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
2 Professor of Sport physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
3 Associate professor of Sport physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
4 Ph.D. of Sport physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Abstract: Alzheimer's disease (AD) is the most common form of dementia and amyloid
peptides playing a central role in its pathogenesis. Recently, regular exercise has been
considered as one of the most important non-pharmacological mechanisms to contrast with
AD. The aim of this study was to investigate the effect of 4 weeks exercise on mRNA of GDNF in rat’s hippocampus with AD induced by Aβ1-42 injection.
Methods: 56 male rats with mean±SD weight of 195±20 g were randomly divided
Into four groups: training group, Aβ-42 induction + training group, Aβ-42 induction group and control group. injection of Aβ1-42 into the hippocampus was performed. Seven days
after surgery, rats of each groups were either sacrificed or subjected to behavioral
testing, randomly. Real-Time PCR were used for investigation of mRNA GDNF.
Results: there is a significant difference between the groups. Gene expression level in training group was higher and in the Aβ-42 induction group was lower (p < 0.001). Moreover, spatial learning and memory were significantly better in the exercise + AD than AD group (p < 0.01).
Conclusion: It seems that 4 weeks of exercise could improve spatial memory and learning. Moreover, aerobic exercise could increase the level of neurotrophic factors gene expression specially GDNF in rat’s hippocampus.

کلیدواژه‌ها [English]

  • Alzheimer's disease
  • Aerobic Exercise
  • GDNF
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