نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزش، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه تربیت‌بدنی، واحد پردیس، دانشگاه آزاد اسلامی، پردیس، ایران.

چکیده

کلروپیریفوس از سموم ارگانوفسفره است که برای کنترل آفات کشاورزی استفاده می‌شود. این سموم باعث ایجاد استرس اکسیداتیو و آپوپتوزیس در بافت­های مختلف بدن از جمله قلب می‌شوند. SERCA2a و NKX2-5 از ژن‌هایی‌اند که تنظیم‌کنندۀ عملکرد انقباضی و رشد قلب هستند. مطالعات پیشین اثر مثبت فعالیت بدنی را بر بیان ژن­های ذکرشده در وضعیت نارسایی قلبی نشان داده‌اند، ولی تاکنون تغییرات این ژن‌ها متعاقب استفاده از فعالیت بدنی در وضعیت مسمومیت با کلروپیریفوس بررسی نشده است؛ ازاین‌رو در پژوهش حاضر به بررسی اثر مستقل و تعاملی تمرین هوازی و مکمل اوژنول بر بیان ژن­های SERCA2a و NKX2-5 در بافت قلب رت‌های مسموم‌شده با کلروپیریفوس پرداخته شده است. تعداد 36 سر رت نر نژاد ویستار به‌ شش گروه (شش سر در هر گروه) با نام­های کنترل سالم، شم، کنترل مسموم، مسموم تمرین هوازی، مسموم اوژنول و مسموم + تمرین هوازی + اوژنول تقسیم شدند. تمرین هوازی روی تردمیل مخصوص جوندگان (پنج جلسه در هفته، هر جلسه 20 دقیقه و با سرعت 26 متر بر دقیقه) به‌مدت چهار هفته اجرا شد. اوژنول با دوز 250 میلی­گرم به‌ازای هر کیلوگرم وزن بدن، پنج روز در هفته و به‌مدت چهار هفته با استفاده از گاواژ به رت­های گروه‌های مکمل خورانده شد. داده­ها با استفاده از آزمون­های تحلیل واریانس یک‌طرفه و تعقیبی توکی تحلیل شدند وP < 0.05  به‌عنوان تغییر معنا­دار در نظر گرفته شد. نتایج نشان داد، مسمومیت به‌طور معنا‌داری بیان ژن‌های SERCA2a و NKX2-5 را در بافت قلب کاهش داد. همچنین تمرین (نه مکمل) بیان ژن‌های SERCA2a و NKX2-5 را در قلب رت­های مسموم افزایش داد. به‌هرحال، تعامل تمرین-اوژنول باعث ایجاد اثر سینرژیک نشد. درمجموع به نظر می­رسد در مطالعۀ حاضر تمرین عاملی مهم‌تر در مقایسه با اوژنول برای کاهش اثر مسمومیت مزمن بر قلب است؛ البته انجام‌دادن مطالعاتی در آینده با دوزهای بیشتر مکمل‌دهی اوژنول نیاز است.

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